Product Description: tubulin--tyrosine ligase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.3.2.25 (Tubulin--tyrosine ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005929 | cilium |
| Function | GO:0015631 | tubulin binding |
| Function | GO:0070740 | tubulin-glutamic acid ligase activity |
| Process | GO:0000226 | microtubule cytoskeleton organization |
| Process | GO:0018095 | protein polyglutamylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.04 | 0.98 |
| Trophozoite | possibly expressed | 0.02 | 0.99 |
| Schizont | expressed | 0.91 | 0.56 |
| Gametocyte | expressed | 0.29 | 0.84 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1009700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.945 | MFS: 0.501 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1208000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1208000)
Modification: Disrupted | RMgm-3204
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJU8
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.38) with 6VZU (Global RMSD=2.36)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.3.2.25 | tubulin-tyrosine ligase | ADPAMPNEML-PheL-TyrL-DopatyraminestathminphosphateL-Tyr-GlyL-Tyr-L-GluL-Ala-L-Tyr... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102355
Most Similar Human Ortholog: F8W9N0
TM-align score: 0.80 | RMSD: 2.89
Seq Identity: 0.32 | Length: 192 / 553
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000170703 | tubulin tyrosine ligase like 6 |
| ENSG00000213471 | tubulin tyrosine ligase like 13, pseudogene |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 0 | 1 | 0 | 3 |
| missense | 0 | 0 | 0 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 0 | 4 | 0 | 1 |
| missense | 0 | 0 | 0 | 0 |
PlasmoDB Total SNPs: 295
Non-coding: 266 | Synonymous: 20 | Nonsynonymous: 9 | Stop Codon: 0
Protein Length: 553 | Molecular Weight (kDa): 65.955
UniProt ID(s): Q8IJU8
PDB ID(s): None
Isoelectric Point: 9.95
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004344 | Tubulin-tyrosine ligase/Tubulin polyglutamylase |
| PFam | PF03133 | Tubulin-tyrosine ligase/Tubulin polyglutamylase |
| Superfamily | SSF56059 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |