PF3D7_1010100

Genome location: Pf3D7_10_v3:403,619..404,723(+)

Genome classification: Core

Function and Localization

Product Description: PI31 domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Process	GO:0006511	ubiquitin-dependent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.49	0.77
Trophozoite	expressed	0.22	0.86
Schizont	expressed	0.12	0.92
Gametocyte	expressed	0.82	0.59

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1010100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.119 | MFS: -2.99 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1208500)

Relative Growth Rate: 0.79 ± 0.17
Confidence: 4.90

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1208500)

Modification: Disrupted | RMgm-3208

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJU4

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104058

Most Similar Human Ortholog: Q5QPM9

TM-align score: 0.73 | RMSD: 2.50

Seq Identity: 0.14 | Length: 156 / 298

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000125818	proteasome inhibitor subunit 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	9	6	26
disruptive	6	31	16	53
missense	6	31	16	51

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	21	10	24
disruptive	11	65	29	79
missense	10	63	26	63

PlasmoDB Total SNPs: 40

Non-coding: 27 | Synonymous: 6 | Nonsynonymous: 7 | Stop Codon: 0

Protein Information

Protein Length: 298 | Molecular Weight (kDa): 35.603

UniProt ID(s): Q8IJU4

PDB ID(s): None

Isoelectric Point: 6.88

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR021625	PI31 proteasome regulator, N-terminal
PFam	PF11566	PI31 proteasome regulator, N-terminal
Superfamily	N/A	N/A