PF3D7_1010200

Genome location: Pf3D7_10_v3:404,903..411,445(-)

Genome classification: Core

Function and Localization

Product Description: DNA2/NAM7 helicase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.12 (DNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Function	GO:0003723	RNA binding
Function	GO:0003724	RNA helicase activity
Process	GO:0000184	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	1.06	0.54
Trophozoite	possibly expressed	0.85	0.57
Schizont	possibly expressed	0.23	0.87
Gametocyte	not expressed	0.60	0.69

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1010200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.132 | MFS: -2.94 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_1208600)

Modification: Other | RMgm-424

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJU3

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.18) with 5MZN (Global RMSD=2.23)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.12	DNA helicase	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101455

Most Similar Human Ortholog: X6RI79

TM-align score: 0.46 | RMSD: 4.02

Seq Identity: 0.30 | Length: 477 / 1839

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000107290	senataxin

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	15	93	50	116
disruptive	86	309	164	367
missense	53	240	136	311

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	29	161	92	137
disruptive	162	547	247	452
missense	96	437	207	341

PlasmoDB Total SNPs: 291

Non-coding: 64 | Synonymous: 135 | Nonsynonymous: 92 | Stop Codon: 0

Protein Information

Protein Length: 1839 | Molecular Weight (kDa): 217.159

UniProt ID(s): Q8IJU3

PDB ID(s): None

Isoelectric Point: 5.83

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
InterPro	IPR041677	DNA2/NAM7 helicase, helicase domain
InterPro	IPR041679	DNA2/NAM7 helicase-like, C-terminal
PFam	PF13086	DNA2/NAM7 helicase, helicase domain
PFam	PF13087	DNA2/NAM7 helicase-like, C-terminal
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase