PF3D7_1011700 (RAD23)

Genome location: Pf3D7_10_v3:448,601..451,031(+)

Genome classification: Core

Function and Localization

Product Description: DNA repair protein RAD23, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005829	cytosol
Component	GO:0005654	nucleoplasm
Function	GO:0003684	damaged DNA binding
Function	GO:0031593	polyubiquitin modification-dependent protein binding
Function	GO:0070628	proteasome binding
Function	GO:0043130	ubiquitin binding
Process	GO:0006289	nucleotide-excision repair
Process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.54	0.75
Trophozoite	expressed	0.81	0.56
Schizont	expressed	0.28	0.81
Gametocyte	expressed	0.62	0.65

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1011700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.756 | MFS: -1.038 | #Insertions: 1

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1210100)

Relative Growth Rate: 0.82 ± 0.10
Confidence: 5.91

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1210100)

Modification: Disrupted | RMgm-3214

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJS8

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101279

Most Similar Human Ortholog: Q5W0S4

TM-align score: 0.60 | RMSD: 2.15

Seq Identity: 0.33 | Length: 57 / 389

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000119318	RAD23 homolog B, nucleotide excision repair protein
ENSG00000179262	RAD23 homolog A, nucleotide excision repair protein

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	22	14	13
disruptive	5	47	29	51
missense	5	45	27	46

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	3	31	21	21
disruptive	12	111	42	121
missense	10	98	31	86

PlasmoDB Total SNPs: 148

Non-coding: 125 | Synonymous: 13 | Nonsynonymous: 10 | Stop Codon: 0

Protein Information

Protein Length: 389 | Molecular Weight (kDa): 44.395

UniProt ID(s): Q8IJS8

PDB ID(s): None

Isoelectric Point: 4.14

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000626	Ubiquitin-like domain
InterPro	IPR004806	UV excision repair protein Rad23
InterPro	IPR009060	UBA-like superfamily
InterPro	IPR015360	XPC-binding domain
InterPro	IPR015940	Ubiquitin-associated domain
InterPro	IPR029071	Ubiquitin-like domain superfamily
InterPro	IPR036353	XPC-binding domain superfamily
PFam	PF00240	Ubiquitin-like domain
PFam	PF00627	Ubiquitin-associated domain
PFam	PF09280	XPC-binding domain
Superfamily	SSF101238	XPC-binding domain superfamily
Superfamily	SSF46934	UBA-like superfamily
Superfamily	SSF54236	Ubiquitin-like domain superfamily

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104