PF3D7_1011800 (PREBP)

Genome location: Pf3D7_10_v3:452,765..459,985(+)

Genome classification: Core

Function and Localization

Product Description: PRE-binding protein

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Function	GO:0003700	DNA-binding transcription factor activity
Function	GO:0003723	RNA binding
Function	GO:0003690	double-stranded DNA binding
Function	GO:0003729	mRNA binding
Process	GO:0010468	regulation of gene expression
Process	GO:0006355	regulation of transcription, DNA-templated

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	2.43	0.16
Trophozoite	expressed	2.32	0.12
Schizont	expressed	1.44	0.33
Gametocyte	expressed	1.61	0.28

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1011800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.125 | MFS: -2.928 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1210200)

Relative Growth Rate: 0.14 ± 0.11
Confidence: 5.81

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJS7

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.86) with 3KO1 (Global RMSD=14.20)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_143929

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	20	79	45	78
disruptive	64	141	82	178
missense	26	102	51	124

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	34	161	49	77
disruptive	115	295	138	292
missense	47	191	95	180

PlasmoDB Total SNPs: 404

Non-coding: 262 | Synonymous: 93 | Nonsynonymous: 49 | Stop Codon: 0

Protein Information

Protein Length: 1139 | Molecular Weight (kDa): 131.626

UniProt ID(s): Q25784, Q8IJS7

PDB ID(s): None

Isoelectric Point: 9.8

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004087	K Homology domain
InterPro	IPR004088	K Homology domain, type 1
InterPro	IPR036612	K Homology domain, type 1 superfamily
PFam	PF00013	K Homology domain, type 1
Superfamily	SSF54791	K Homology domain, type 1 superfamily

Associated Publications

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108
24040327	Identification of a novel and unique transcription factor in theintraerythrocytic stage of Plasmodium falciparum.	Komaki-Yasuda K, Okuwaki M, ..., Kano S	10.1371/journal.pone.0074701
30379851	Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation.	Briquet S, Ourimi A, ..., Vaquero C	10.1371/journal.pone.0205596
33906926	Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH	Sinha A, Baumgarten S, ..., Scherf A	10.1128/mBio.00661-21
30902138	PRE-binding protein of Plasmodium falciparum is a potential candidate for vaccinedesign and development: An in silico evaluation of the hypothesis	Sanasam BD, Kumar S	10.1016/j.mehy.2019.01.006
38128025	The RNA-binding KH-domain in the unique transcription factor of the malariaparasite is responsible for its transcriptional regulatory activity	Komaki-Yasuda K, Kano S	10.1371/journal.pone.0296165