PF3D7_1011900 (HO)

Genome location: Pf3D7_10_v3:460,036..462,335(-)

Genome classification: Core

Function and Localization

Product Description: heme oxygenase

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 1.14.14.18 (Heme oxygenase (biliverdin-producing))

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0004392	heme oxygenase (decyclizing) activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.09	0.96
Trophozoite	expressed	0.54	0.68
Schizont	expressed	0.69	0.59
Gametocyte	expressed	0.63	0.65

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1011900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.853 | MFS: 0 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1210300)

Relative Growth Rate: 0.11 ± 0.17
Confidence: 4.99

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJS6

"Best" AlphaFill ligand hit: HEM (heme, Local RMSD=1.02) with 7CKA (Global RMSD=2.37)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
1.14.14.18	heme oxygenase (biliverdin-producing)	COKCNDTTDTEEDTAazideU0126SL327cyanidecysteineFR180204azalanstat...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_156412

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	14	9	25
disruptive	5	22	15	41
missense	5	21	15	30

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	4	27	19	23
disruptive	9	60	41	71
missense	6	47	30	48

PlasmoDB Total SNPs: 142

Non-coding: 132 | Synonymous: 9 | Nonsynonymous: 1 | Stop Codon: 0

Protein Information

Protein Length: 305 | Molecular Weight (kDa): 37.012

UniProt ID(s): Q8IJS6

PDB ID(s): None

Isoelectric Point: 9.68

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR016084	Haem oxygenase-like, multi-helical
PFam	N/A	N/A
Superfamily	SSF48613	Haem oxygenase-like, multi-helical

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
19073183	The novel heme oxygenase-like protein from Plasmodiumfalciparum converts heme tobilirubin IXalpha in the apicoplast.	Okada K	10.1016/j.febslet.2008.12.015
22992734	Direct tests of enzymatic heme degradation by the malaria parasite Plasmodiumfalciparum.	Sigala PA, Crowley JR, ..., Goldberg DE	10.1074/jbc.M112.414078