PF3D7_1012000

Genome location: Pf3D7_10_v3:462,633..467,542(+)

Genome classification: Core

Function and Localization

Product Description: RING zinc finger protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 6.3.2.19 (Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0000976	transcription cis-regulatory region binding
Process	GO:0045944	positive regulation of transcription by RNA polymerase II

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.14	0.93
Trophozoite	expressed	0.41	0.75
Schizont	expressed	0.81	0.55
Gametocyte	expressed	0.60	0.67

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1012000

Resistome Missense Mutations: Y440D

Resistome Compounds with Missense Mutations: BI-2536

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.731 | #Insertions: 4

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1210400)

Relative Growth Rate: 1.03 ± 0.09
Confidence: 6.25

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1210400)

Modification: Disrupted | RMgm-3217

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJS5

"Best" AlphaFill ligand hit: TYD (thymidine-5'-diphosphate, Local RMSD=1.60) with 5TPV (Global RMSD=17.61)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
6.3.2.19	ubiquitin-protein ligase	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102933

Most Similar Human Ortholog: H0YFQ9

TM-align score: 0.54 | RMSD: 3.84

Seq Identity: 0.20 | Length: 149 / 781

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000022840	ring finger protein 10
ENSG00000288623	novel protein

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	10	42	25	52
disruptive	13	84	45	128
missense	11	80	43	120

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	17	77	31	56
disruptive	28	178	82	178
missense	17	165	72	143

PlasmoDB Total SNPs: 251

Non-coding: 216 | Synonymous: 24 | Nonsynonymous: 11 | Stop Codon: 0

Protein Information

Protein Length: 781 | Molecular Weight (kDa): 93.482

UniProt ID(s): Q8IJS5

PDB ID(s): None

Isoelectric Point: 9.26

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001841	Zinc finger, RING-type
InterPro	IPR017907	Zinc finger, RING-type, conserved site
InterPro	IPR018957	Zinc finger, C3HC4 RING-type
InterPro	IPR039739	RING-finger protein Mag2/Rnf10
PFam	PF00097	Zinc finger, C3HC4 RING-type
Superfamily	SSF57850	N/A