Product Description: hypoxanthine-guanine phosphoribosyltransferase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.4.2.8 (Hypoxanthine phosphoribosyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0003723 | RNA binding |
| Function | GO:0004422 | hypoxanthine phosphoribosyltransferase activity |
| Function | GO:0000287 | magnesium ion binding |
| Process | GO:0032263 | GMP salvage |
| Process | GO:0032264 | IMP salvage |
| Process | GO:0006168 | adenine salvage |
| Process | GO:0006178 | guanine salvage |
| Process | GO:0046100 | hypoxanthine metabolic process |
| Process | GO:0006166 | purine ribonucleoside salvage |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.41 | 0.52 |
| Trophozoite | expressed | 3.23 | 0.09 |
| Schizont | expressed | 0.55 | 0.72 |
| Gametocyte | expressed | 0.71 | 0.66 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1012400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.15 | MFS: -2.897 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1210800)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1210800)
Modification: Disrupted | RMgm-3221
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJS1
"Best" AlphaFill ligand hit: HPA (hypoxanthine, Local RMSD=0.23) with 3OZF (Global RMSD=0.90)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.4.2.8 | hypoxanthine phosphoribosyltransferase | ATPADPUDPAMPGTPGDPKClGMPCDPITPIMPIDP... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P00492 | Hypoxanthine-guanine phosphoribosyltransferase | Homo sapiens | HOGENOM, OMA, OrthoDB, OrthoMCL BLAST | HGXPRT Inhibitor, 2HGXPRT Inhibitor, 3US9200020, Table 1, Compound 1US9200020, Table 1, Compound 2... |
| P9WHQ9 | Hypoxanthine-guanine phosphoribosyltransferase | OMA, OrthoMCL, OrthoMCL BLAST | CHEMBL4282830CHEMBL4294529CHEMBL4277753CHEMBL4291117... | |
| A0A0J9TE27 | Hypoxanthine phosphoribosyltransferase | OrthoMCL BLAST | US9200020, Table 3 compound 1US9200020, Table 3 compound 2US9200020, Table 3 compound 5US9200020, Table 3 compound 6... | |
| P20035 | Hypoxanthine-guanine-xanthine phosphoribosyltransferase | Plasmodium falciparum | OrthoMCL BLAST | HGXPRT Inhibitor, 2HGXPRT Inhibitor, 3US9200020, Table 1, Compound 1US9200020, Table 1, Compound 1B... |
Ortholog Group (OrthoMCL): OG6_101078
Most Similar Human Ortholog: Q9NRG1
TM-align score: 0.92 | RMSD: 1.35
Seq Identity: 0.44 | Length: 215 / 231
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000099256 | phosphoribosyl transferase domain containing 1 |
| ENSG00000165704 | hypoxanthine phosphoribosyltransferase 1 |
Protein Length: 231 | Molecular Weight (kDa): 26.362
PDB ID(s): None
Isoelectric Point: 7.66
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000836 | Phosphoribosyltransferase domain |
| InterPro | IPR005904 | Hypoxanthine phosphoribosyl transferase |
| InterPro | IPR029057 | Phosphoribosyltransferase-like |
| PFam | PF00156 | Phosphoribosyltransferase domain |
| Superfamily | SSF53271 | Phosphoribosyltransferase-like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 18 | 8 | 9 |
| disruptive | 1 | 1 | 2 | 4 |
| missense | 0 | 0 | 0 | 2 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 26 | 10 | 15 |
| disruptive | 1 | 6 | 3 | 19 |
| missense | 0 | 1 | 1 | 14 |
PlasmoDB Total SNPs: 157
Non-coding: 153 | Synonymous: 3 | Nonsynonymous: 0 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 2205541 | Cloning and expression in Escherichia coli of a hypoxanthine-guaninephosphoribosyltransferase-encoding cDNA from Plasmodium falciparum. | Vasanthakumar G, Davis RL Jr, Sullivan MA, Donahue JP | 10.1016/0378-1119(90)90163-l |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 31536893 | Sulfide, sulfoxide and sulfone bridged acyclic nucleoside phosphonates asinhibitors of the Plasmodium falciparum and human 6-oxopurinephosphoribosyltransferases: Synthesis and evaluation. | Klejch T, Keough DT, ..., Hockova D | 10.1016/j.ejmech.2019.111667 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 38404541 | Targeting XGHPRT enzyme to manage Helicobacter pylori induced gastric cancer: Amulti-pronged machine learning, artificial intelligence and biophysics-basedstudy | Alabbas AB | 10.1016/j.sjbs.2024.103960 |
| 37810145 | Hypoxanthine guanine phosphoribosyltransferase 1, a target of miR-125b-5p,promotes cell proliferation and invasion in head and neck squamous cellcarcinoma | Yuan L, Xiao Z, Lu R | 10.1016/j.heliyon.2023.e20174 |