PF3D7_1012500

Genome location: Pf3D7_10_v3:477,806..481,170(-)

Genome classification: Core

Function and Localization

Product Description: phosphoglucomutase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 5.4.2.10 (Phosphoglucosamine mutase);5.4.2.2 (Phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent))

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.04	0.98
Trophozoite	expressed	0.11	0.93
Schizont	expressed	0.02	0.98
Gametocyte	expressed	0.12	0.92

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1012500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.779 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1210900)

Relative Growth Rate: 0.99 ± 0.24
Confidence: 4.27

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1210900)

Modification: Disrupted | RMgm-3222

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJS0

"Best" AlphaFill ligand hit: G6Q (glucose-6-phosphate, Local RMSD=0.65) with 4HJH (Global RMSD=2.92)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
5.4.2.10	phosphoglucosamine mutase	EDTA
5.4.2.2	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	ATPAMPGTPCTPUTPEDTACaCl2CoCl2NiCl2citrateisocitratecis-aconitate...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100912

Most Similar Human Ortholog: Q6PCE3

TM-align score: 0.96 | RMSD: 1.49

Seq Identity: 0.38 | Length: 586 / 593

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000165434	phosphoglucomutase 2 like 1
ENSG00000169299	phosphoglucomutase 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	32	15	40
disruptive	8	29	18	38
missense	8	27	18	36

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	72	21	44
disruptive	15	65	24	73
missense	12	55	24	60

PlasmoDB Total SNPs: 185

Non-coding: 154 | Synonymous: 15 | Nonsynonymous: 16 | Stop Codon: 0

Protein Information

Protein Length: 593 | Molecular Weight (kDa): 68.3

UniProt ID(s): Q8IJS0

PDB ID(s): None

Isoelectric Point: 6.79

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR005843	Alpha-D-phosphohexomutase, C-terminal
InterPro	IPR005844	Alpha-D-phosphohexomutase, alpha/beta/alpha domain I
InterPro	IPR005845	Alpha-D-phosphohexomutase, alpha/beta/alpha domain II
InterPro	IPR005846	Alpha-D-phosphohexomutase, alpha/beta/alpha domain III
InterPro	IPR016055	Alpha-D-phosphohexomutase, alpha/beta/alpha I/II/III
InterPro	IPR016066	Alpha-D-phosphohexomutase, conserved site
InterPro	IPR036900	Alpha-D-phosphohexomutase, C-terminal domain superfamily
PFam	PF00408	Alpha-D-phosphohexomutase, C-terminal
PFam	PF02878	Alpha-D-phosphohexomutase, alpha/beta/alpha domain I
PFam	PF02879	Alpha-D-phosphohexomutase, alpha/beta/alpha domain II
PFam	PF02880	Alpha-D-phosphohexomutase, alpha/beta/alpha domain III
Superfamily	SSF53738	Alpha-D-phosphohexomutase, alpha/beta/alpha I/II/III
Superfamily	SSF55957	Alpha-D-phosphohexomutase, C-terminal domain superfamily