Product Description: NLI interacting factor-like phosphatase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.3.16 (Protein-serine/threonine phosphatase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Function | GO:0008420 | RNA polymerase II CTD heptapeptide repeat phosphatase activity |
| Process | GO:0070940 | dephosphorylation of RNA polymerase II C-terminal domain |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.36 | 0.82 |
| Trophozoite | not expressed | 0.70 | 0.62 |
| Schizont | possibly expressed | 0.58 | 0.66 |
| Gametocyte | not expressed | 0.45 | 0.75 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1012700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.143 | MFS: -2.771 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1211100)
Modification: Tagged | RMgm-1091
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJR8
"Best" AlphaFill ligand hit: 1PG (2-(2-{2-[2-(2-methoxy-ethoxy)-ethoxy]-ethoxy}-ethoxy)-ethanol, Local RMSD=0.33) with 3L0B (Global RMSD=15.75)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.3.16 | protein-serine/threonine phosphatase | ATPADPUDPAMPGTPUTPUMPNaFEDTAEGTAcAMPNADPH... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 73 | 53 | 93 |
| disruptive | 44 | 208 | 134 | 287 |
| missense | 31 | 186 | 124 | 245 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 22 | 152 | 70 | 102 |
| disruptive | 84 | 468 | 205 | 434 |
| missense | 62 | 384 | 173 | 296 |
PlasmoDB Total SNPs: 306
Non-coding: 156 | Synonymous: 77 | Nonsynonymous: 73 | Stop Codon: 0
Protein Length: 1438 | Molecular Weight (kDa): 171.012
UniProt ID(s): Q8IJR8
PDB ID(s): None
Isoelectric Point: 4.85
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004274 | FCP1 homology domain |
| InterPro | IPR036412 | HAD-like superfamily |
| InterPro | IPR039189 | CTD phosphatase Fcp1 |
| PFam | PF03031 | FCP1 homology domain |
| Superfamily | SSF56784 | HAD-like superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 25011111 | Genome-wide functional analysis of Plasmodium protein phosphatases reveals keyregulators of parasite development and differentiation. | Guttery DS, Poulin B, ..., Tewari R | 10.1016/j.chom.2014.05.020 |
| 27694982 | Genome-wide association analysis identifies genetic loci associated withresistance to multiple antimalarials in Plasmodium falciparum from China-Myanmarborder. | Wang Z, Cabrera M, ..., Cui L | 10.1038/srep33891 |
| 35938722 | The Plasmodium falciparum Nuclear Protein Phosphatase NIF4 Is Required forEfficient Merozoite Invasion and Regulates Artemisinin Sensitivity | Zhu X, Li S, ..., Cao Y | 10.1128/mbio.01897-22 |