Product Description: autophagy-related protein 18
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0031410 | cytoplasmic vesicle |
| Component | GO:0005829 | cytosol |
| Component | GO:0019898 | extrinsic component of membrane |
| Component | GO:0020020 | food vacuole |
| Component | GO:0034045 | phagophore assembly site membrane |
| Function | GO:0080025 | phosphatidylinositol-3,5-bisphosphate binding |
| Function | GO:0032266 | phosphatidylinositol-3-phosphate binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0000045 | autophagosome assembly |
| Process | GO:0000422 | autophagy of mitochondrion |
| Process | GO:0044804 | autophagy of nucleus |
| Process | GO:0043572 | plastid fission |
| Process | GO:0006497 | protein lipidation |
| Process | GO:0034497 | protein localization to phagophore assembly site |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.50 | 0.75 |
| Trophozoite | expressed | 0.41 | 0.75 |
| Schizont | expressed | 0.22 | 0.84 |
| Gametocyte | expressed | 0.22 | 0.87 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1012900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.141 | MFS: -3.265 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1211300)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJR6
"Best" AlphaFill ligand hit: CMP (cyclic amp, Local RMSD=12.56) with 6EZU (Global RMSD=16.62)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102082
Most Similar Human Ortholog: Q5MNZ6
TM-align score: 0.89 | RMSD: 2.39
Seq Identity: 0.33 | Length: 330 / 380
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000141580 | WD repeat domain 45B |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 0 | 1 | 0 | 1 |
| missense | 0 | 0 | 0 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 0 | 3 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
PlasmoDB Total SNPs: 247
Non-coding: 229 | Synonymous: 17 | Nonsynonymous: 1 | Stop Codon: 0
Protein Length: 380 | Molecular Weight (kDa): 43.52
UniProt ID(s): Q8IJR6
PDB ID(s): None
Isoelectric Point: 9.61
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001680 | WD40 repeat |
| InterPro | IPR036322 | WD40-repeat-containing domain superfamily |
| PFam | N/A | N/A |
| Superfamily | SSF50978 | WD40-repeat-containing domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 27694982 | Genome-wide association analysis identifies genetic loci associated withresistance to multiple antimalarials in Plasmodium falciparum from China-Myanmarborder. | Wang Z, Cabrera M, ..., Cui L | 10.1038/srep33891 |
| 29089429 | Autophagy-Related Protein A TG18 Regulates Apicoplast Biogenesis in Apicomplexan Parasites | Bansal P, Tripathi A, ..., Sharma P | 10.1128/mBio.01468-17 |
| 30367653 | A single nucleotide polymorphism in the Plasmodium falciparum atg18 geneassociates with artemisinin resistance and confers enhanced parasite survivalunder nutrient deprivation. | Breglio KF, Amato R, ..., Simon AK | 10.1186/s12936-018-2532-x |
| 31863491 | Autophagy-related protein PfATG18 participates in food vacuole dynamics andautophagy-like pathway in Plasmodium falciparum. | Agrawal P, Manjithaya R, Surolia N | 10.1111/mmi.14441 |
| 33843972 | Plasmodium falciparum Atg18 localizes to the food vacuole via interaction withthe multi-drug resistance protein 1 and phosphatidylinositol 3-phosphate. | Sudhakar R, Das D, ..., Sijwali PS | 10.1042/BCJ20210001 |
| 36803233 | Atg1-dependent phosphorylation of Vps34 is required for dynamic regulation of thephagophore assembly site and autophagy in Saccharomyces cerevisiae | Lee Y, Kim B, Jang HS, Huh WK | 10.1080/15548627.2023.2182478 |
| 35420484 | Autophagy Underlies the Proteostasis Mechanisms of Artemisinin Resistance in P.falciparum Malaria | Ray A, Mathur M, ..., Surolia N | 10.1128/mbio.00630-22 |
| 37139661 | Autophagic and non-autophagic functions of the Saccharomyces cerevisiae PROPPINsAtg18, Atg21 and Hsv2 | Marquardt L, Thumm M | 10.1515/hsz-2023-0126 |