About Gene List

PF3D7_1014100 (MSA180)

Genome location: Pf3D7_10_v3:554,233..560,227(+)

Genome classification: Core

Function and Localization

Product Description: merozoite surface protein MSA180

SignalP Peptide: MNRIFYFCLFTILFWLSLVSG

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0009986 cell surface
Component GO:0005737 cytoplasm
Component GO:1903561 extracellular vesicle
Component GO:0020003 symbiont-containing vacuole
Function GO:0005515 protein binding
Process GO:0044409 entry into host
Process GO:0035891 exit from host cell

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.08 0.96
Trophozoite possibly expressed 0.04 0.98
Schizont expressed 1.02 0.67
Gametocyte not expressed 0.13 0.93

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1014100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -2.967 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJQ4

"Best" AlphaFill ligand hit: NAP (nadp nicotinamide-adenine-dinucleotide phosphate, Local RMSD=9.31) with 4DPD (Global RMSD=25.54)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533342

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 4 36 36 69
disruptive 32 108 76 164
missense 26 106 70 148

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 7 89 54 101
disruptive 58 229 134 255
missense 45 201 122 203

PlasmoDB Total SNPs: 201

Non-coding: 89 | Synonymous: 59 | Nonsynonymous: 51 | Stop Codon: 2

Protein Information

Protein Length: 1455 | Molecular Weight (kDa): 173.276

UniProt ID(s): Q8IJQ4

PDB ID(s): None

Isoelectric Point: 6

Protein Domain Annotations:

Source Family ID Description
InterPro N/A N/A
PFam N/A N/A
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
26292257 Discovery of Novel Plasmodium falciparum Pre-Erythrocytic Antigens for VaccineDevelopment. Aguiar JC, Bolton J, ..., Richie TL 10.1371/journal.pone.0136109
28351409 Identification of a novel merozoite surface antigen of Plasmodium vivax,PvMSA180. Muh F, Han JH, ..., Han ET 10.1186/s12936-017-1760-9
28944300 Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. Abdi A, Yu L, ..., Rayner J 10.12688/wellcomeopenres.11910.2
30976034 PfMSA180 is a novel Plasmodium falciparum vaccine antigen that interacts withhuman erythrocyte integrin associated protein (CD47). Nagaoka H, Sasaoka C, ..., Takashima E 10.1038/s41598-019-42366-9
33932049 Autocatalytic activation of a malarial egress protease is druggable and requiresa protein cofactor. Tan MSY, Koussis K, ..., Blackman MJ 10.15252/embj.2020107226
34584166 Genetic disruption of Plasmodium falciparum Merozoite surface antigen 180(PfMSA180) suggests an essential role during parasite egress from erythrocytes. Bahl V, Chaddha K, ..., Gaur D 10.1038/s41598-021-98707-0