PF3D7_1014200 (HAP2)

Genome location: Pf3D7_10_v3:560,538..563,353(+)

Genome classification: Core

Function and Localization

Product Description: male gamete fusion factor HAP2

SignalP Peptide: N/A

# Transmembrane Domains: 2

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005929	cilium

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.00	1.00
Trophozoite	not expressed	0.00	1.00
Schizont	not expressed	0.01	0.99
Gametocyte	possibly expressed	0.11	0.93

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1014200

Resistome Missense Mutations: L193F

Resistome Compounds with Missense Mutations: MMV665794

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.876 | MFS: -2.845 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1212600)

Relative Growth Rate: 1.01 ± 0.15
Confidence: 5.15

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1212600)

Modification: Disrupted | RMgm-5344

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJQ3

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_154402

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	25	17	38
disruptive	6	48	25	90
missense	6	47	23	83

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	56	30	34
disruptive	23	85	52	119
missense	14	82	44	98

PlasmoDB Total SNPs: 70

Non-coding: 10 | Synonymous: 45 | Nonsynonymous: 15 | Stop Codon: 0

Protein Information

Protein Length: 889 | Molecular Weight (kDa): 102.627

UniProt ID(s): Q8IJQ3, W8P8N4, W8R6N4, W8S2H7

PDB ID(s): None

Isoelectric Point: 9.5

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR018928	Generative cell specific-1/HAP2 domain
InterPro	IPR040326	HAP2/GCS1
PFam	PF10699	Generative cell specific-1/HAP2 domain
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
38071314	Evaluation of a new fusion antigen, cd loop and HAP2-GCS1 domain (cd-HAP) ofPlasmodium falciparum Generative Cell Specific 1 antigen formulated with variousadjuvants, as a transmission blocking vaccine	Pourhashem Z, Nourani L, ..., Mehrizi AA	10.1186/s12936-023-04798-7