PF3D7_1014300

Genome location: Pf3D7_10_v3:564,313..573,464(+)

Genome classification: Core

Function and Localization

Product Description: SPRY domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 8

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0016020	membrane
Component	GO:0005634	nucleus
Process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.13	0.94
Trophozoite	expressed	0.95	0.53
Schizont	expressed	0.69	0.64
Gametocyte	expressed	1.47	0.35

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1014300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.901 | #Insertions: 5

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1212700)

Modification: Disrupted | RMgm-260

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJQ2

"Best" AlphaFill ligand hit: AN2 (amp phosphoramidate, Local RMSD=4.20) with 3IED (Global RMSD=27.79)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_118075

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	16	83	65	129
disruptive	64	314	185	400
missense	37	253	148	313

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	29	182	85	135
disruptive	141	627	272	535
missense	83	471	214	409

PlasmoDB Total SNPs: 270

Non-coding: 73 | Synonymous: 122 | Nonsynonymous: 75 | Stop Codon: 0

Protein Information

Protein Length: 2379 | Molecular Weight (kDa): 283.131

UniProt ID(s): Q8IJQ2

PDB ID(s): None

Isoelectric Point: 6.67

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR003877	SPRY domain
InterPro	IPR013144	CRA domain
InterPro	IPR013320	Concanavalin A-like lectin/glucanase domain superfamily
PFam	PF00622	SPRY domain
Superfamily	SSF49899	Concanavalin A-like lectin/glucanase domain superfamily

PMID	Title	Authors	DOI/Link
11152613	Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes.	Krogh A, Larsson B, von Heijne G, Sonnhammer EL	10.1006/jmbi.2000.4315
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097