PF3D7_1014800

Genome location: Pf3D7_10_v3:596,497..600,413(-)

Genome classification: Core

Function and Localization

Product Description: EF hand domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.04	0.98
Trophozoite	not expressed	0.27	0.86
Schizont	not expressed	0.27	0.84
Gametocyte	not expressed	0.45	0.77

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1014800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.999 | MFS: -1.827 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1213200)

Relative Growth Rate: 0.85 ± 0.19
Confidence: 4.74

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1213200)

Modification: Disrupted | RMgm-3238

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJP7

"Best" AlphaFill ligand hit: PLP (pyridoxal-5'-phosphate, Local RMSD=10.85) with 3K7Y (Global RMSD=16.06)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_144656

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	39	23	48
disruptive	10	65	26	124
missense	10	62	24	119

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	58	38	44
disruptive	27	135	66	143
missense	17	115	61	122

PlasmoDB Total SNPs: 192

Non-coding: 154 | Synonymous: 30 | Nonsynonymous: 8 | Stop Codon: 0

Protein Information

Protein Length: 834 | Molecular Weight (kDa): 100.746

UniProt ID(s): Q8IJP7

PDB ID(s): None

Isoelectric Point: 6.78

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR011992	EF-hand domain pair
InterPro	IPR025663	A-kinase anchor protein 28kDa
PFam	PF14469	A-kinase anchor protein 28kDa
Superfamily	SSF47473	EF-hand domain pair