Product Description: cysteine--tRNA ligase
SignalP Peptide: MNLFLFICIALFIYLDK
# Transmembrane Domains: 0
EC Numbers: 6.1.1.16 (Cysteine--tRNA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Function | GO:0005524 | ATP binding |
| Function | GO:0004817 | cysteine-tRNA ligase activity |
| Process | GO:0006423 | cysteinyl-tRNA aminoacylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | nd | nd |
| Trophozoite | expressed | nd | nd |
| Schizont | expressed | nd | nd |
| Gametocyte | possibly expressed | nd | nd |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1015200.3
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.267 | MFS: -2.689 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A143ZZ50
"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.65) with 3SP1 (Global RMSD=3.54)
BRENDA EC Inhibitors:
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P9WJM9 | L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase | Mycobacterium tuberculosis | OrthoMCL BLAST | CysSA |
Ortholog Group (OrthoMCL): OG6_100332
Most Similar Human Ortholog: B4DKY1
TM-align score: 0.75 | RMSD: 2.51
Seq Identity: 0.42 | Length: 534 / 677
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000110619 | cysteinyl-tRNA synthetase 1 |
| ENSG00000134905 | cysteinyl-tRNA synthetase 2, mitochondrial |
| ENSG00000278191 | cysteinyl-tRNA synthetase 1 |
Protein Length: 677 | Molecular Weight (kDa): 79.505
UniProt ID(s): Q8IJP3
PDB ID(s): None
Isoelectric Point: 9.14
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR009080 | Aminoacyl-tRNA synthetase, class Ia, anticodon-binding |
| InterPro | IPR015803 | Cysteine-tRNA ligase |
| InterPro | IPR024909 | Cysteinyl-tRNA synthetase/mycothiol ligase |
| InterPro | IPR032678 | tRNA synthetases class I, catalytic domain |
| PFam | PF01406 | tRNA synthetases class I, catalytic domain |
| Superfamily | SSF47323 | Aminoacyl-tRNA synthetase, class Ia, anticodon-binding |
| Superfamily | SSF52374 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 32 | 14 | 35 |
| disruptive | 2 | 47 | 24 | 78 |
| missense | 2 | 46 | 24 | 71 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 71 | 26 | 28 |
| disruptive | 15 | 128 | 57 | 107 |
| missense | 10 | 100 | 50 | 79 |
PlasmoDB Total SNPs: 48
Non-coding: 29 | Synonymous: 12 | Nonsynonymous: 7 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 24428730 | A dual-targeted aminoacyl-tRNA synthetase in Plasmodium falciparum chargescytosolic and apicoplast tRNACys | Pham JS, Sakaguchi R, ..., Ralph SA | 10.1042/BJ20131451 |