Genome location: Pf3D7_10_v3:617,544..620,659(+)
Genome classification: Core
Product Description: methionine aminopeptidase 1b, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.11.18 (Methionyl aminopeptidase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Function | GO:0008235 | metalloexopeptidase activity |
| Process | GO:0006464 | cellular protein modification process |
| Process | GO:0006508 | proteolysis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.46 | 0.78 |
| Trophozoite | expressed | 0.60 | 0.68 |
| Schizont | expressed | 0.07 | 0.96 |
| Gametocyte | expressed | 0.35 | 0.80 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1015300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.936 | #Insertions: 3
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1213700)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1213700)
Modification: Disrupted | RMgm-3241
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJP2
"Best" AlphaFill ligand hit: HED (2-hydroxyethyl disulfide, Local RMSD=0.30) with 4IDY (Global RMSD=1.55)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.11.18 | methionyl aminopeptidase | BRENDA SOAP query unsuccessful |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q01662 | Methionine aminopeptidase 1 | Saccharomyces cerevisiae | OrthoDB, OrthoMCL | CHEMBL360513CHEMBL359541CHEMBL89671CHEMBL313650... |
| P53582 | Methionine aminopeptidase 1 | Homo sapiens | OrthoDB | bestatin-type inhibitor, 4bestatin-type inhibitor, 5bestatin-type inhibitor, 8CHEMBL327579... |
| P0AE18 | Methionine aminopeptidase | Escherichia coli | OrthoMCL | 3,5-Pyrazolediamine Inhibitor, 33,5-Pyrazolediamine Inhibitor, 43,5-Pyrazolediamine Inhibitor, 53,5-Pyrazolediamine Inhibitor, 6... |
Ortholog Group (OrthoMCL): OG6_100342
Most Similar Human Ortholog: H0Y955
TM-align score: 0.92 | RMSD: 1.97
Seq Identity: 0.56 | Length: 166 / 517
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000164024 | methionyl aminopeptidase 1 |
| ENSG00000172878 | methionyl aminopeptidase type 1D, mitochondrial |
Protein Length: 517 | Molecular Weight (kDa): 59.724
UniProt ID(s): Q8IJP2
PDB ID(s): 3S6B
Isoelectric Point: 7.11
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000994 | Peptidase M24 |
| InterPro | IPR001714 | Peptidase M24, methionine aminopeptidase |
| InterPro | IPR002467 | Peptidase M24A, methionine aminopeptidase, subfamily 1 |
| InterPro | IPR031615 | MYND-like zinc finger, mRNA-binding |
| InterPro | IPR036005 | Creatinase/aminopeptidase-like |
| PFam | PF00557 | Peptidase M24 |
| PFam | PF15801 | MYND-like zinc finger, mRNA-binding |
| Superfamily | SSF55920 | Creatinase/aminopeptidase-like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 26 | 13 | 36 |
| disruptive | 13 | 49 | 24 | 67 |
| missense | 5 | 46 | 24 | 54 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 57 | 19 | 30 |
| disruptive | 23 | 114 | 41 | 106 |
| missense | 13 | 93 | 32 | 64 |
PlasmoDB Total SNPs: 121
Non-coding: 73 | Synonymous: 27 | Nonsynonymous: 20 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |