PF3D7_1015800

Genome location: Pf3D7_10_v3:632,488..635,500(+)

Genome classification: Core

Function and Localization

Product Description: ribonucleoside-diphosphate reductase small chain, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 1.17.4.1 (Ribonucleoside-diphosphate reductase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005971	ribonucleoside-diphosphate reductase complex
Function	GO:0004748	ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor
Process	GO:0006260	DNA replication
Process	GO:0009186	deoxyribonucleoside diphosphate metabolic process
Process	GO:0009263	deoxyribonucleotide biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.06	0.97
Trophozoite	expressed	0.56	0.67
Schizont	expressed	0.27	0.82
Gametocyte	possibly expressed	0.37	0.78

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1015800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.13 | MFS: -3.167 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1214200)

Relative Growth Rate: 0.14 ± 0.15
Confidence: 5.20

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJN8

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
1.17.4.1	ribonucleoside-diphosphate reductase	BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_156441

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	11	5	17
disruptive	3	9	7	16
missense	3	9	7	13

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	22	14	22
disruptive	6	25	22	44
missense	5	22	18	29

PlasmoDB Total SNPs: 145

Non-coding: 123 | Synonymous: 16 | Nonsynonymous: 6 | Stop Codon: 0

Protein Information

Protein Length: 335 | Molecular Weight (kDa): 40.388

UniProt ID(s): Q6BBQ6, Q8IJN8

PDB ID(s): None

Isoelectric Point: 5.2

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000358	Ribonucleotide reductase small subunit family
InterPro	IPR009078	Ferritin-like superfamily
InterPro	IPR033909	Ribonucleotide reductase small subunit
PFam	PF00268	Ribonucleotide reductase small subunit family
Superfamily	SSF47240	Ferritin-like superfamily