Product Description: enolase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 4.2.1.11 (Phosphopyruvate hydratase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0009986 | cell surface |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005856 | cytoskeleton |
| Component | GO:0020020 | food vacuole |
| Component | GO:0005634 | nucleus |
| Component | GO:0000015 | phosphopyruvate hydratase complex |
| Component | GO:0005886 | plasma membrane |
| Function | GO:0003723 | RNA binding |
| Function | GO:0098808 | mRNA cap binding |
| Function | GO:0004634 | phosphopyruvate hydratase activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0002253 | activation of immune response |
| Process | GO:0000045 | autophagosome assembly |
| Process | GO:0006096 | glycolytic process |
| Process | GO:0032889 | regulation of vacuole fusion, non-autophagic |
| Process | GO:0006351 | transcription, DNA-templated |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.92 | 0.62 |
| Trophozoite | expressed | 1.78 | 0.28 |
| Schizont | expressed | 0.23 | 0.87 |
| Gametocyte | expressed | 0.59 | 0.70 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1015900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.121 | MFS: -2.917 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1214300)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJN7
"Best" AlphaFill ligand hit: 0V5 ((2r)-2-(phosphonooxy)propanoic acid, Local RMSD=0.36) with 3UJS (Global RMSD=1.01)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 4.2.1.11 | phosphopyruvate hydratase | KClKBrEDTAMgCl2CaCl2SO42-PO43-NaClO4p19rascitrateNa2PO3Ffluoride... |
Orthology to BindingDB Entries:
Ortholog Group (OrthoMCL): OG6_100260
Most Similar Human Ortholog: P09104
TM-align score: 0.98 | RMSD: 0.75
Seq Identity: 0.70 | Length: 431 / 446
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000074800 | enolase 1 |
| ENSG00000108515 | enolase 3 |
| ENSG00000111674 | enolase 2 |
Protein Length: 446 | Molecular Weight (kDa): 48.678
UniProt ID(s): A0A143ZZ61, Q27727, Q8IJN7
PDB ID(s): None
Isoelectric Point: 6.51
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000941 | Enolase |
| InterPro | IPR020809 | Enolase, conserved site |
| InterPro | IPR020810 | Enolase, C-terminal TIM barrel domain |
| InterPro | IPR020811 | Enolase, N-terminal |
| InterPro | IPR029017 | Enolase-like, N-terminal |
| InterPro | IPR036849 | Enolase-like, C-terminal domain superfamily |
| PFam | PF00113 | Enolase, C-terminal TIM barrel domain |
| PFam | PF03952 | Enolase, N-terminal |
| Superfamily | SSF51604 | Enolase-like, C-terminal domain superfamily |
| Superfamily | SSF54826 | Enolase-like, N-terminal |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 17 | 8 | 19 |
| disruptive | 2 | 9 | 7 | 17 |
| missense | 2 | 9 | 7 | 15 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 33 | 12 | 16 |
| disruptive | 7 | 18 | 16 | 42 |
| missense | 6 | 15 | 14 | 30 |
PlasmoDB Total SNPs: 80
Non-coding: 70 | Synonymous: 8 | Nonsynonymous: 1 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 17785475 | Protective properties and surface localization of Plasmodium falciparum enolase. | Pal-Bhowmick I, Mehta M, ..., Jarori GK | 10.1128/IAI.00551-07 |
| 19642995 | Plasmodium falciparum enolase: stage-specific expression and sub-cellularlocalization. | Bhowmick IP, Kumar N, ..., Jarori GK | 10.1186/1475-2875-8-179 |
| 24474798 | Multiple pathways for Plasmodium ookinete invasion of the mosquito midgut. | Vega-Rodriguez J, Ghosh AK, ..., Jacobs-Lorena M | 10.1073/pnas.1315517111 |
| 24494818 | A secretory multifunctional serine protease, DegP of Plasmodium falciparum, playsan important role in thermo-oxidative stress, parasite growth and development. | Sharma S, Jadli M, ..., Malhotra P | 10.1111/febs.12732 |
| 26341815 | Cloning, expression, purification and characterization of Plasmodium spp.glyceraldehyde-3-phosphate dehydrogenase. | Sangolgi PB, Balaji C, ..., Jarori GK | 10.1016/j.pep.2015.08.028 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |