PF3D7_1017300 (GRASP)

Genome location: Pf3D7_10_v3:694,276..696,787(-)

Genome classification: Core

Function and Localization

Product Description: golgi re-assembly stacking protein 1, golgi re-assembly stacking protein 2

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005794	Golgi apparatus
Component	GO:0005801	cis-Golgi network
Process	GO:0007030	Golgi organization

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	nd	nd
Trophozoite	possibly expressed	nd	nd
Schizont	possibly expressed	nd	nd
Gametocyte	expressed	nd	nd

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1017300.2

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.849 | #Insertions: 3

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJM6

"Best" AlphaFill ligand hit: EPH (l-alpha-phosphatidyl-beta-oleoyl-gamma-palmitoyl-phosphatidylethanolamine, Local RMSD=4.88) with 5Z9O (Global RMSD=12.35)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102404

Most Similar Human Ortholog: C9J9V9

TM-align score: 0.70 | RMSD: 1.82

Seq Identity: 0.28 | Length: 71 / 573

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000114745	golgi reassembly stacking protein 1
ENSG00000115806	golgi reassembly stacking protein 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	37	17	39
disruptive	16	72	40	94
missense	15	71	37	88

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	68	19	37
disruptive	27	149	81	143
missense	25	136	69	117

PlasmoDB Total SNPs: 64

Non-coding: 15 | Synonymous: 29 | Nonsynonymous: 20 | Stop Codon: 0

Protein Information

Protein Length: 573 | Molecular Weight (kDa): 66.839

UniProt ID(s): C6S3C8

PDB ID(s): None

Isoelectric Point: 4.73

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007583	GRASP55/65
InterPro	IPR024958	GRASP-type PDZ domain
InterPro	IPR036034	PDZ superfamily
PFam	PF04495	GRASP-type PDZ domain
Superfamily	SSF50156	PDZ superfamily