About Gene List

PF3D7_1017400 (HAD5)

Genome location: Pf3D7_10_v3:697,137..700,274(-)

Genome classification: Core

Function and Localization

Product Description: phosphomannomutase

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 5.4.2.8 (Phosphomannomutase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005829 cytosol
Function GO:0004614 phosphoglucomutase activity
Function GO:0004615 phosphomannomutase activity
Process GO:0009298 GDP-mannose biosynthetic process
Process GO:0006506 GPI anchor biosynthetic process
Process GO:0044409 entry into host
Process GO:0035891 exit from host cell
Process GO:0006013 mannose metabolic process
Process GO:0006487 protein N-linked glycosylation
Process GO:0045047 protein targeting to ER

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 0.43 0.80
Trophozoite expressed 0.34 0.80
Schizont expressed 0.11 0.93
Gametocyte expressed 0.61 0.67

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1017400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.121 | MFS: -3.116 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0501700)

  • Relative Growth Rate: 0.95 ± 0.07
  • Confidence: 6.77

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0501700)

Modification: Disrupted | RMgm-4616

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJM5

"Best" AlphaFill ligand hit: IMP (inosinic acid, Local RMSD=1.87) with 6CFV (Global RMSD=2.73)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
5.4.2.8 phosphomannomutase ATPADPIMPvanadateclodronateUDP-D-glucoseDisperse Blue 56glucose 6-phosphateglucose 1-phosphatemannose 1-phosphatefructose 1-phosphateD-xylose 1-phosphate...

Orthology to BindingDB Entries:

UniProt Protein Name Species Homology Source BindingDB Ligands
O15305 Phosphomannomutase 2 Homo sapiens OMA, OrthoDB med.21724, Compound 153cid_8033400cid_1510390727664-91-7...

Orthology Information

Ortholog Group (OrthoMCL): OG6_101634

Most Similar Human Ortholog: O15305

TM-align score: 0.95 | RMSD: 1.17

Seq Identity: 0.52 | Length: 240 / 244

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000100417 phosphomannomutase 1
ENSG00000140650 phosphomannomutase 2

Protein Information

Protein Length: 244 | Molecular Weight (kDa): 28.597

UniProt ID(s): Q8IJM5

PDB ID(s): 7MYV

Isoelectric Point: 7.65

Protein Domain Annotations:

Source Family ID Description
InterPro IPR005002 Phosphomannomutase
InterPro IPR006379 HAD-superfamily hydrolase, subfamily IIB
InterPro IPR036412 HAD-like superfamily
PFam PF03332 Phosphomannomutase
Superfamily SSF56784 HAD-like superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 5 8 4 11
disruptive 2 13 3 11
missense 2 13 2 10

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 6 13 14 19
disruptive 8 21 19 48
missense 6 17 15 39

PlasmoDB Total SNPs: 185

Non-coding: 165 | Synonymous: 14 | Nonsynonymous: 6 | Stop Codon: 0

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
34973333 Enzymatic and structural characterization of HAD5, an essentialphosphomannomutase of malaria-causing parasites. Frasse PM, Miller JJ, ..., Odom John AR 10.1016/j.jbc.2021.101550