Product Description: 26S proteasome regulatory subunit p55, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.25.1 (Proteasome endopeptidase complex)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0031595 | nuclear proteasome complex |
| Component | GO:0005634 | nucleus |
| Component | GO:0005838 | proteasome regulatory particle |
| Component | GO:0008541 | proteasome regulatory particle, lid subcomplex |
| Function | GO:0004175 | endopeptidase activity |
| Process | GO:0043161 | proteasome-mediated ubiquitin-dependent protein catabolic process |
| Process | GO:0006511 | ubiquitin-dependent protein catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.41 | 0.81 |
| Trophozoite | expressed | 1.06 | 0.46 |
| Schizont | expressed | 0.69 | 0.61 |
| Gametocyte | expressed | 0.62 | 0.65 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1017900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.143 | MFS: -3.288 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0502200)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJM0
"Best" AlphaFill ligand hit: UDP (uridine-5'-diphosphate, Local RMSD=2.31) with 6OQ7 (Global RMSD=21.47)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.25.1 | proteasome endopeptidase complex | ATPSDSPSIEDTANH4+EGTATLCKALLNNMDANLVSPI 1PA28... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102014
Most Similar Human Ortholog: O00232
TM-align score: 0.91 | RMSD: 2.04
Seq Identity: 0.39 | Length: 438 / 467
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000197170 | proteasome 26S subunit, non-ATPase 12 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 21 | 9 | 24 |
| disruptive | 6 | 26 | 10 | 49 |
| missense | 6 | 26 | 10 | 41 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 42 | 15 | 27 |
| disruptive | 18 | 46 | 31 | 91 |
| missense | 11 | 42 | 28 | 63 |
PlasmoDB Total SNPs: 148
Non-coding: 125 | Synonymous: 15 | Nonsynonymous: 8 | Stop Codon: 0
Protein Length: 467 | Molecular Weight (kDa): 55.209
UniProt ID(s): Q8IJM0
PDB ID(s): None
Isoelectric Point: 5.6
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000717 | Proteasome component (PCI) domain |
| InterPro | IPR035297 | 26S Proteasome non-ATPase regulatory subunit 12 |
| InterPro | IPR036390 | Winged helix DNA-binding domain superfamily |
| InterPro | IPR040134 | 26S Proteasome non-ATPase regulatory subunit 12/COP9 signalosome complex subunit 4 |
| InterPro | IPR040896 | 26S proteasome regulatory subunit RPN5, C-terminal domain |
| PFam | PF01399 | Proteasome component (PCI) domain |
| PFam | PF18098 | 26S proteasome regulatory subunit RPN5, C-terminal domain |
| Superfamily | SSF46785 | Winged helix DNA-binding domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30229448 | NMR (1)H, (13)C, (15)N backbone and side chain resonance assignment of theN-terminal domain of yeast proteasome lid subunit Rpn5 | Zhang W, Zhao C, Hu Y, Jin C | 10.1007/s12104-018-9840-5 |
| 17761670 | Isolation of the Schizosaccharomyces pombe proteasome subunit Rpn7 and astructure-function study of the proteasome-COP9-initiation factor domain | Sha Z, Yen HC, ..., Chang EC | 10.1074/jbc.M706276200 |
| 20471955 | Dissection of the assembly pathway of the proteasome lid in Saccharomycescerevisiae | Fukunaga K, Kudo T, ..., Saeki Y | 10.1016/j.bbrc.2010.05.061 |
| 17135287 | The assembly pathway of the 19S regulatory particle of the yeast 26S proteasome | Isono E, Nishihara K, ..., Toh-e A | 10.1091/mbc.e06-07-0635 |
| 25631053 | Solution structure of yeast Rpn9: insights into proteasome lid assembly | Hu Y, Wu Y, ..., Jin C | 10.1074/jbc.M114.626762 |