Product Description: tRNA pseudouridine synthase, putative
SignalP Peptide: MVIILLLAIICSYNITS
# Transmembrane Domains: 0
EC Numbers: 5.4.99.12 (tRNA pseudouridine(38-40) synthase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0009982 | pseudouridine synthase activity |
| Function | GO:0004730 | pseudouridylate synthase activity |
| Process | GO:0006396 | RNA processing |
| Process | GO:1990481 | mRNA pseudouridine synthesis |
| Process | GO:0006400 | tRNA modification |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.13 | 0.94 |
| Trophozoite | not expressed | 0.49 | 0.71 |
| Schizont | not expressed | 0.12 | 0.92 |
| Gametocyte | not expressed | 0.32 | 0.81 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1018000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.712 | MFS: -3.154 | #Insertions: 1
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1025200)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0502300)
Modification: Disrupted | RMgm-1939
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I715
"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=8.41) with 2HVY (Global RMSD=5.89)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 5.4.99.12 | tRNA pseudouridine38-40 synthase | tRNApoly(rA)npoly(rC)npoly(rG)npoly(rI)npoly(rU)niodoacetateCalf thymus DNADithionitrobenzoate5-fluorouracil tRNAPhep-chloromercuribenzoatefluorouracil-substituted tRNA |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100327
Most Similar Human Ortholog: Q8WWH5
TM-align score: 0.66 | RMSD: 2.66
Seq Identity: 0.31 | Length: 248 / 560
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000130826 | dyskerin pseudouridine synthase 1 |
| ENSG00000165832 | TruB pseudouridine synthase family member 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 20 | 22 | 37 |
| disruptive | 24 | 77 | 36 | 85 |
| missense | 13 | 64 | 33 | 63 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 56 | 23 | 37 |
| disruptive | 46 | 173 | 63 | 150 |
| missense | 26 | 121 | 45 | 90 |
PlasmoDB Total SNPs: 58
Non-coding: 9 | Synonymous: 29 | Nonsynonymous: 20 | Stop Codon: 0
Protein Length: 560 | Molecular Weight (kDa): 66.373
UniProt ID(s): Q8I715
PDB ID(s): None
Isoelectric Point: 10.16
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002501 | Pseudouridine synthase II, N-terminal |
| InterPro | IPR014780 | tRNA pseudouridine synthase II, TruB |
| InterPro | IPR020103 | Pseudouridine synthase, catalytic domain superfamily |
| PFam | PF01509 | Pseudouridine synthase II, N-terminal |
| Superfamily | SSF55120 | Pseudouridine synthase, catalytic domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |