Product Description: pseudophosphatase PPP8
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.3.16 (Protein-serine/threonine phosphatase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0045178 | basal part of cell |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Component | GO:0032991 | protein-containing complex |
| Function | GO:0005515 | protein binding |
| Function | GO:0004722 | protein serine/threonine phosphatase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | no data | N/A | N/A |
| Ring | no data | 0.19 | 0.91 |
| Trophozoite | no data | 0.40 | 0.79 |
| Schizont | no data | 2.27 | 0.28 |
| Gametocyte | no data | 0.98 | 0.56 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1018200
Resistome Missense Mutations: D1941N
Resistome Compounds with Missense Mutations: MMV982556
Resistome # Samples with Disruptive Mutations: 2 (2 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.243 | MFS: -2.701 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0502500)
Modification: Disrupted | RMgm-1940
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6S3C9
"Best" AlphaFill ligand hit: NHE (2-[n-cyclohexylamino]ethane sulfonic acid, Local RMSD=0.20) with 6GHM (Global RMSD=3.10)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.3.16 | protein-serine/threonine phosphatase | ATPADPUDPAMPGTPUTPUMPNaFEDTAEGTAcAMPNADPH... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 17 | 91 | 52 | 98 |
| disruptive | 104 | 256 | 158 | 311 |
| missense | 64 | 210 | 146 | 278 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 32 | 159 | 68 | 147 |
| disruptive | 162 | 518 | 230 | 438 |
| missense | 98 | 431 | 200 | 361 |
PlasmoDB Total SNPs: 264
Non-coding: 55 | Synonymous: 84 | Nonsynonymous: 125 | Stop Codon: 0
Protein Length: 2166 | Molecular Weight (kDa): 254.109
UniProt ID(s): C6S3C9
PDB ID(s): None
Isoelectric Point: 5.62
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006186 | Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase |
| InterPro | IPR011992 | EF-hand domain pair |
| InterPro | IPR018247 | EF-Hand 1, calcium-binding site |
| PFam | N/A | N/A |
| Superfamily | SSF47473 | EF-hand domain pair |
| Superfamily | SSF56300 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 25011111 | Genome-wide functional analysis of Plasmodium protein phosphatases reveals keyregulators of parasite development and differentiation. | Guttery DS, Poulin B, ..., Tewari R | 10.1016/j.chom.2014.05.020 |
| 31097714 | An essential contractile ring protein controls cell division in Plasmodiumfalciparum. | Rudlaff RM, Kraemer S, Streva VA, Dvorin JD | 10.1038/s41467-019-10214-z |