PF3D7_1019000

Genome location: Pf3D7_10_v3:756,646..765,280(+)

Genome classification: Core

Function and Localization

Product Description: eukaryotic translation initiation factor subunit eIF2A, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0022627	cytosolic small ribosomal subunit
Component	GO:0005634	nucleus
Function	GO:0003729	mRNA binding
Function	GO:0043022	ribosome binding
Function	GO:0000049	tRNA binding
Function	GO:0003743	translation initiation factor activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	1.73	0.31
Trophozoite	expressed	1.46	0.33
Schizont	expressed	1.62	0.26
Gametocyte	possibly expressed	0.90	0.54

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1019000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 3 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.17 | MFS: -2.758 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0503200)

Relative Growth Rate: 0.41 ± 0.34
Confidence: 3.56

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJL2

"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=2.80) with 6JWQ (Global RMSD=9.97)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_164672

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	28	91	53	167
disruptive	137	261	172	476
missense	61	189	139	404

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	48	181	99	171
disruptive	224	590	306	672
missense	98	442	246	462

PlasmoDB Total SNPs: 504

Non-coding: 145 | Synonymous: 180 | Nonsynonymous: 179 | Stop Codon: 0

Protein Information

Protein Length: 2405 | Molecular Weight (kDa): 280.755

UniProt ID(s): Q8IJL2

PDB ID(s): None

Isoelectric Point: 8.96

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR003169	GYF domain
InterPro	IPR011387	Translation initiation factor 2A
InterPro	IPR013979	Translation initiation factor, beta propellor-like domain
InterPro	IPR035445	GYF-like domain superfamily
PFam	PF02213	GYF domain
PFam	PF08662	Translation initiation factor, beta propellor-like domain
Superfamily	SSF55277	GYF-like domain superfamily
Superfamily	SSF82171

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108
27381095	The mRNA-bound proteome of the human malaria parasite Plasmodium falciparum.	Bunnik EM, Batugedara G, ..., Le Roch KG	10.1186/s13059-016-1014-0