PF3D7_1019500

Genome location: Pf3D7_10_v3:787,427..795,435(-)

Genome classification: Core

Function and Localization

Product Description: TMEM94 domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 10

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	nd	nd
Trophozoite	possibly expressed	nd	nd
Schizont	expressed	nd	nd
Gametocyte	possibly expressed	nd	nd

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1019500.2

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.993 | MFS: -2.415 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0503700)

Relative Growth Rate: 0.97 ± 0.07
Confidence: 6.68

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0503700)

Modification: Disrupted | RMgm-1950

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A143ZZS2

"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=4.54) with 4ZCF (Global RMSD=14.63)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104175

Most Similar Human Ortholog: J3QRY7

TM-align score: 0.82 | RMSD: 3.21

Seq Identity: 0.18 | Length: 283 / 1241

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000177728	transmembrane protein 94

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	13	63	43	71
disruptive	6	45	33	102
missense	6	45	33	89

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	23	119	59	93
disruptive	23	146	61	198
missense	12	118	50	159

PlasmoDB Total SNPs: 543

Non-coding: 470 | Synonymous: 60 | Nonsynonymous: 13 | Stop Codon: 0

Protein Information

Protein Length: 1241 | Molecular Weight (kDa): 147.203

UniProt ID(s): A0A143ZXG9

PDB ID(s): None

Isoelectric Point: 9.36

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR023298	P-type ATPase, transmembrane domain superfamily
InterPro	IPR023299	P-type ATPase, cytoplasmic domain N
InterPro	IPR039720	Transmembrane protein 94
PFam	N/A	N/A
Superfamily	SSF81660	P-type ATPase, cytoplasmic domain N
Superfamily	SSF81665	P-type ATPase, transmembrane domain superfamily