Product Description: tRNA-specific 2-thiouridylase MnmA
SignalP Peptide: N/A
# Transmembrane Domains: 3
EC Numbers: 2.1.1.61 (tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.12 | 0.94 |
| Trophozoite | expressed | 0.74 | 0.60 |
| Schizont | expressed | 0.89 | 0.52 |
| Gametocyte | expressed | 0.76 | 0.62 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1019800
Resistome Missense Mutations: N619K
Resistome Compounds with Missense Mutations: AN13762
Resistome # Samples with Disruptive Mutations: 3 (3 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.221 | MFS: -2.671 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0504000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJK4
"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.52) with 2DEU (Global RMSD=4.15)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.1.1.61 | tRNA 5-(aminomethyl)-2-thiouridylate-methyltransferase | p-chloromercuribenzoate |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101163
Most Similar Human Ortholog: A0A2R8Y4R7
TM-align score: 0.83 | RMSD: 2.80
Seq Identity: 0.25 | Length: 311 / 1084
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000100416 | tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 40 | 13 | 53 |
| disruptive | 31 | 133 | 57 | 178 |
| missense | 21 | 90 | 49 | 126 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 15 | 72 | 51 | 82 |
| disruptive | 101 | 300 | 133 | 334 |
| missense | 47 | 187 | 91 | 220 |
PlasmoDB Total SNPs: 148
Non-coding: 0 | Synonymous: 114 | Nonsynonymous: 34 | Stop Codon: 0
Protein Length: 1084 | Molecular Weight (kDa): 130.518
UniProt ID(s): Q8IJK4
PDB ID(s): None
Isoelectric Point: 10.22
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | N/A | N/A |
| PFam | PF03054 | N/A |
| Superfamily | SSF52402 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 36865327 | A tRNA modification in Mycobacterium tuberculosis facilitates optimal intracellular growth | Tomasi FG, Kimura S, Rubin EJ, Waldor MK | 10.1101/2023.02.20.529267 |
| 37166116 | The Plasmodium falciparum apicoplast cysteine desulfurase provides sulfur forboth iron-sulfur cluster assembly and tRNA modification | Swift RP, Elahi R, ..., Prigge ST | 10.7554/eLife.84491 |
| 35467390 | Sulfur Availability Impacts Accumulation of the 2-Thiouridine tRNA Modificationin Bacillus subtilis | Edwards AM, Black KA, Dos Santos PC | 10.1128/jb.00009-22 |
| 30450093 | Recent Advances in Our Understanding of the Biosynthesis of Sulfur Modificationsin tRNAs | Shigi N | 10.3389/fmicb.2018.02679 |
| 37755167 | A tRNA modification in Mycobacterium tuberculosis facilitates optimal intracellular growth | Tomasi FG, Kimura S, Rubin EJ, Waldor MK | 10.7554/eLife.87146 |