Product Description: autophagy-related protein 8
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0031410 | cytoplasmic vesicle |
| Component | GO:0005829 | cytosol |
| Component | GO:0016020 | membrane |
| Component | GO:0005634 | nucleus |
| Function | GO:0005515 | protein binding |
| Process | GO:0006995 | cellular response to nitrogen starvation |
| Process | GO:0042493 | response to drug |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.02 | 0.99 |
| Trophozoite | expressed | 0.08 | 0.95 |
| Schizont | expressed | 0.37 | 0.79 |
| Gametocyte | expressed | 0.18 | 0.89 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1019900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.282 | MFS: -2.603 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0504100)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0504100)
Modification: Mutated | RMgm-1476
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJK2
"Best" AlphaFill ligand hit: OXE (ortho-xylene, Local RMSD=0.17) with 7ZKR (Global RMSD=1.82)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| D7RA28 | GABA(A) receptor-associated protein | Cavia cutleri | OMA, OrthoDB | CHEMBL278777CHEMBL306806CCK antagonist synthetic 22 |
| Q9H492 | Microtubule-associated proteins 1A/1B light chain 3A | OrthoDB | NOVOBIOCINCHEMBL4851364CHEMBL4878719CHEMBL4861964... | |
| Q9GZQ8 | Microtubule-associated proteins 1A/1B light chain 3B | OrthoDB | US11021457, Compound 61US11021457, Compound 2US11021457, Compound 3US11021457, Compound 4... |
Ortholog Group (OrthoMCL): OG6_100707
Most Similar Human Ortholog: P60520
TM-align score: 0.93 | RMSD: 1.07
Seq Identity: 0.43 | Length: 113 / 124
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000034713 | GABA type A receptor associated protein like 2 |
| ENSG00000139112 | GABA type A receptor associated protein like 1 |
| ENSG00000170296 | GABA type A receptor-associated protein |
Protein Length: 124 | Molecular Weight (kDa): 14.65
UniProt ID(s): Q8IJK2
PDB ID(s): 4EOY
Isoelectric Point: 8.24
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004241 | Autophagy protein Atg8 ubiquitin-like |
| InterPro | IPR029071 | Ubiquitin-like domain superfamily |
| PFam | PF02991 | Autophagy protein Atg8 ubiquitin-like |
| Superfamily | SSF54236 | Ubiquitin-like domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 2 | 3 | 1 | 0 |
| missense | 0 | 0 | 0 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 3 | 3 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
PlasmoDB Total SNPs: 120
Non-coding: 118 | Synonymous: 1 | Nonsynonymous: 1 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 22819059 | Autophagy in Apicomplexa: a life sustaining death mechanism? | Sinai AP, Roepe PD | 10.1016/j.pt.2012.06.006 |
| 22900071 | Autophagy-related Atg8 localizes to the apicoplast of the human malaria parasitePlasmodium falciparum. | Kitamura K, Kishi-Itakura C, ..., Mizushima N | 10.1371/journal.pone.0042977 |
| 22982544 | Structural characterization and inhibition of the Plasmodium Atg8-Atg3interaction. | Hain AU, Weltzer RR, ..., Bosch J | 10.1016/j.jsb.2012.09.001 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 24275162 | The multifunctional autophagy pathway in the human malaria parasite, Plasmodiumfalciparum. | Cervantes S, Bunnik EM, ..., Le Roch KG | 10.4161/auto.26743 |
| 24786226 | Identification of an Atg8-Atg3 protein-protein interaction inhibitor from themedicines for Malaria Venture Malaria Box active in blood and liver stagePlasmodium falciparum parasites. | Hain AU, Bartee D, ..., Bosch J | 10.1021/jm401675a |
| 25426852 | Characterization of the autophagy marker protein Atg8 reveals atypical featuresof autophagy in Plasmodium falciparum. | Navale R, Atul, Allanki AD, Sijwali PS | 10.1371/journal.pone.0113220 |
| 38258560 | Effect of ATG8 or SAC1 deficiency on the cell proliferation and lifespan of thelong-lived PMT1 deficiency yeast cells | Cui H, Cui X, ..., Liu X | 10.1093/femsle/fnad121 |