PF3D7_1020400 (RCM1)

Genome location: Pf3D7_10_v3:822,220..824,901(-)

Genome classification: Core

Function and Localization

Product Description: rRNA (cytosine-C(5))-methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.- (Methyltransferases.);2.1.1.12 (Methionine S-methyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005730	nucleolus
Function	GO:0009383	rRNA (cytosine-C5-)-methyltransferase activity
Process	GO:0001510	RNA methylation
Process	GO:0070475	rRNA base methylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.55	0.74
Trophozoite	expressed	0.51	0.72
Schizont	expressed	0.12	0.93
Gametocyte	expressed	0.67	0.64

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1020400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.999 | MFS: -2.032 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJJ8

"Best" AlphaFill ligand hit: SFG (sinefungin, Local RMSD=0.85) with 2YXL (Global RMSD=2.28)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.-	Ex: homocysteine S-methyltransferase	EDTACTABNickelA9145CtylosintacrineTween 80FTI-2628macrocinadenosineRelomycinphosphate...
2.1.1.12	methionine S-methyltransferase	NEMD-MetGly-MetL-ethionineL-methioninolN-formyl-L-MetL-MethioninamideL-Met ethyl esterL-Met methyl esterseleno-L-methionineL-Met p-nitroanilideS-methyl-L-methionine...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102591

Most Similar Human Ortholog: Q96P11

TM-align score: 0.79 | RMSD: 3.25

Seq Identity: 0.20 | Length: 388 / 601

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000130305	NOP2/Sun RNA methyltransferase 5

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	7	25	18	41
disruptive	12	72	31	106
missense	11	64	27	96

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	11	52	29	39
disruptive	31	143	72	166
missense	24	117	63	111

PlasmoDB Total SNPs: 168

Non-coding: 112 | Synonymous: 36 | Nonsynonymous: 20 | Stop Codon: 0

Protein Information

Protein Length: 601 | Molecular Weight (kDa): 70.77

UniProt ID(s): Q8IJJ8

PDB ID(s): None

Isoelectric Point: 9.48

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001678	SAM-dependent methyltransferase RsmB/NOP2-type
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
PFam	PF01189	SAM-dependent methyltransferase RsmB/NOP2-type
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase