PF3D7_1020500

Genome location: Pf3D7_10_v3:825,330..826,971(+)

Genome classification: Core

Function and Localization

Product Description: ribosomal silencing factor RsfS, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion
Function	GO:0043023	ribosomal large subunit binding
Process	GO:0090071	negative regulation of ribosome biogenesis
Process	GO:0017148	negative regulation of translation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.09	0.95
Trophozoite	expressed	0.36	0.78
Schizont	expressed	0.08	0.95
Gametocyte	expressed	0.39	0.78

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1020500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.389 | MFS: -2.719 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJJ7

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_129401

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	7	20	11	32
disruptive	11	64	34	70
missense	6	45	28	62

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	11	41	22	31
disruptive	31	119	68	131
missense	14	94	59	103

PlasmoDB Total SNPs: 89

Non-coding: 32 | Synonymous: 41 | Nonsynonymous: 16 | Stop Codon: 0

Protein Information

Protein Length: 506 | Molecular Weight (kDa): 60.198

UniProt ID(s): Q8IJJ7

PDB ID(s): None

Isoelectric Point: 5.31

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004394	Protein Iojap/ribosomal silencing factor RsfS
InterPro	IPR043519	Nucleotidyltransferase superfamily
PFam	PF02410	N/A
Superfamily	SSF81301	Nucleotidyltransferase superfamily

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
34494883	A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology.	van Esveld SL, Meerstein-Kessel L, ..., Huynen MA	10.1128/mSphere.00614-21