Product Description: dihydrolipoamide acyltransferase component E2
SignalP Peptide: MLYNLIILIFYLRFSKCIS
# Transmembrane Domains: 0
EC Numbers: 2.3.1.- (Transferring groups other than amino-acyl groups.);2.3.1.12 (Dihydrolipoyllysine-residue acetyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Function | GO:0004742 | dihydrolipoyllysine-residue acetyltransferase activity |
| Process | GO:0006633 | fatty acid biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.10 | 0.95 |
| Trophozoite | expressed | 0.08 | 0.95 |
| Schizont | possibly expressed | 0.04 | 0.97 |
| Gametocyte | expressed | 0.40 | 0.77 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1020800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.136 | MFS: -2.875 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0505000)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0505000)
Modification: Disrupted | RMgm-4618
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJJ4
"Best" AlphaFill ligand hit: CAO (oxidized coenzyme a, Local RMSD=0.11) with 2II3 (Global RMSD=2.76)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.1.- | Ex: [myelin-proteolipid] O-palmitoyltransferase | CoAKClArgATPADPEDTAMgCl2CaCl2MnCl2AgNO3CoCl2FeSO4... |
| 2.3.1.12 | dihydrolipoyllysine-residue acetyltransferase | CoAIodinelipoamidasepalmitoyl-CoAN-Acetylimidazoleguanidine hydrochlorideS-acetyldihydrolipoamide1,2-diselenolane-3-valeric acid |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101258
Most Similar Human Ortholog: A0A7P0TA47
TM-align score: 0.49 | RMSD: 2.10
Seq Identity: 0.28 | Length: 227 / 640
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000150768 | dihydrolipoamide S-acetyltransferase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 22 | 20 | 46 |
| disruptive | 4 | 41 | 32 | 94 |
| missense | 4 | 41 | 32 | 91 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 57 | 39 | 42 |
| disruptive | 9 | 119 | 84 | 140 |
| missense | 8 | 101 | 73 | 96 |
PlasmoDB Total SNPs: 226
Non-coding: 199 | Synonymous: 14 | Nonsynonymous: 13 | Stop Codon: 0
Protein Length: 640 | Molecular Weight (kDa): 73.89
PDB ID(s): None
Isoelectric Point: 6.77
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000089 | Biotin/lipoyl attachment |
| InterPro | IPR001078 | 2-oxoacid dehydrogenase acyltransferase, catalytic domain |
| InterPro | IPR003016 | 2-oxo acid dehydrogenase, lipoyl-binding site |
| InterPro | IPR011053 | Single hybrid motif |
| PFam | PF00198 | 2-oxoacid dehydrogenase acyltransferase, catalytic domain |
| PFam | PF00364 | Biotin/lipoyl attachment |
| Superfamily | SSF51230 | Single hybrid motif |
| Superfamily | SSF52777 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 15612915 | The malaria parasite Plasmodium falciparum has only one pyruvate dehydrogenasecomplex, which is located in the apicoplast. | Foth BJ, Stimmler LM, ..., McFadden GI | 10.1111/j.1365-2958.2004.04407.x |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |