Product Description: ADP-ribosylation factor 1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005794 | Golgi apparatus |
| Component | GO:0036396 | RNA N6-methyladenosine methyltransferase complex |
| Component | GO:0005801 | cis-Golgi network |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0020020 | food vacuole |
| Function | GO:0005525 | GTP binding |
| Function | GO:0003924 | GTPase activity |
| Function | GO:1990583 | phospholipase D activator activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0006886 | intracellular protein transport |
| Process | GO:0006471 | protein ADP-ribosylation |
| Process | GO:0015031 | protein transport |
| Process | GO:0016192 | vesicle-mediated transport |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.51 | 0.76 |
| Trophozoite | expressed | 1.26 | 0.39 |
| Schizont | expressed | 1.18 | 0.45 |
| Gametocyte | expressed | 0.75 | 0.59 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1020900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.251 | MFS: -3.264 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0505100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q94650
"Best" AlphaFill ligand hit: GTP (guanosine-5'-triphosphate, Local RMSD=0.05) with 2H57 (Global RMSD=1.58)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100600
Most Similar Human Ortholog: P61204
TM-align score: 0.94 | RMSD: 1.32
Seq Identity: 0.76 | Length: 175 / 181
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000134287 | ADP ribosylation factor 3 |
| ENSG00000143761 | ADP ribosylation factor 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 2 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 2 | 0 | 0 | 1 |
| missense | 0 | 0 | 0 | 0 |
PlasmoDB Total SNPs: 165
Non-coding: 157 | Synonymous: 6 | Nonsynonymous: 1 | Stop Codon: 1
Protein Length: 181 | Molecular Weight (kDa): 20.912
UniProt ID(s): A0A143ZY58, Q7KQL3, Q94650
PDB ID(s): 3LRP
Isoelectric Point: 6.11
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR005225 | Small GTP-binding protein domain |
| InterPro | IPR006689 | Small GTPase superfamily, ARF/SAR type |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00025 | Small GTPase superfamily, ARF/SAR type |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 8954160 | Isolation, expression and characterization of the gene for an ADP-ribosylationfactor from the human malaria parasite, Plasmodium falciparum. | Stafford WH, Stockley RW, Ludbrook SB, Holder AA | 10.1111/j.1432-1033.1996.0104r.x |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 19171150 | A unique phosphatidylinositol 4-phosphate 5-kinase is activated byADP-ribosylation factor in Plasmodium falciparum. | Leber W, Skippen A, ..., Baker DA | 10.1016/j.ijpara.2008.11.015 |
| 21045287 | Structure of Plasmodium falciparum ADP-ribosylation factor 1. | Cook WJ, Smith CD, ..., Chattopadhyay D | 10.1107/S1744309110036997 |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 25909331 | Alternative Protein Secretion in the Malaria Parasite Plasmodium falciparum. | Thavayogarajah T, Gangopadhyay P, ..., Holder AA | 10.1371/journal.pone.0125191 |
| 31384004 | Transcriptome-wide dynamics of extensive m(6)A mRNA methylation during Plasmodiumfalciparum blood-stage development. | Baumgarten S, Bryant JM, ..., Scherf A | 10.1038/s41564-019-0521-7 |
| 33604307 | Rab5b-Associated Arf1 GTPase Regulates Export of N-Myristoylated Adenylate Kinase2 From the Endoplasmic Reticulum in Plasmodium falciparum. | Taku I, Hirai T, ..., Saito-Nakano Y | 10.3389/fcimb.2020.610200 |
| 38349168 | Toxoplasma and Plasmodium associate with host Arfs during infection | Schroeder EA, Toro-Moreno M, ..., Derbyshire ER | 10.1128/msphere.00770-23 |
| 38014604 | Arf1 GTPase Regulates Golgi-Dependent G2/M Transition and Spindle Organization in Oocyte Meiosis | Zhang KH, Zou YJ, ..., Sun SC | 10.1002/advs.202303009 |
| 38055815 | Immunity-linked genes are stimulated by a membrane stress pathway linked to Golgi function and the ARF-1 GTPase | Fanelli MJ, Welsh CM, ..., Walker AK | 10.1126/sciadv.adi5545 |
| 37989735 | Myr-Arf1 conformational flexibility at the membrane surface sheds light on the interactions with ArfGAP ASAP1 | Zhang Y, Soubias O, ..., Byrd RA | 10.1038/s41467-023-43008-5 |