PF3D7_1021500 (ROK1)

Genome location: Pf3D7_10_v3:867,337..870,148(-)

Genome classification: Core

Function and Localization

Product Description: ATP-dependent RNA helicase ROK1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast
Function	GO:0003724	RNA helicase activity
Process	GO:0030490	maturation of SSU-rRNA

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.71	0.67
Trophozoite	expressed	0.67	0.63
Schizont	expressed	0.20	0.87
Gametocyte	expressed	0.98	0.52

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1021500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.126 | MFS: -3.27 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0505700)

Relative Growth Rate: 0.76 ± 0.10
Confidence: 6.09

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0505700)

Modification: Disrupted | RMgm-1959

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJI8

"Best" AlphaFill ligand hit: CXS (3-cyclohexyl-1-propylsulfonic acid, Local RMSD=0.08) with 2WAX (Global RMSD=4.55)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.13	RNA helicase	ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102417

Most Similar Human Ortholog: Q9Y2R4

TM-align score: 0.69 | RMSD: 3.66

Seq Identity: 0.29 | Length: 459 / 680

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000277594	DExD-box helicase 52
ENSG00000278053	DExD-box helicase 52

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	30	13	45
disruptive	17	82	43	137
missense	7	64	37	107

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	59	30	43
disruptive	40	184	116	222
missense	19	133	101	148

PlasmoDB Total SNPs: 61

Non-coding: 16 | Synonymous: 30 | Nonsynonymous: 15 | Stop Codon: 0

Protein Information

Protein Length: 680 | Molecular Weight (kDa): 79.862

UniProt ID(s): Q8IJI8

PDB ID(s): None

Isoelectric Point: 10

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR011545	DEAD/DEAH box helicase domain
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00270	DEAD/DEAH box helicase domain
PFam	PF00271	Helicase, C-terminal
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

Associated Publications

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
22144892	A systematic screen to discover and analyze apicoplast proteins identifies aconserved and essential protein import factor.	Sheiner L, Demerly JL, ..., Striepen B	10.1371/journal.ppat.1002392
26310304	Identification of Psk2, Skp1, and Tub4 as trans-acting factors foruORF-containing ROK1 mRNA in Saccharomyces cerevisiae	Jeon S, Lim S, Ha J, Kim J	10.1007/s12275-015-5389-5
20969870	Upstream open reading frames regulate the cell cycle-dependent expression of theRNA helicase Rok1 in Saccharomyces cerevisiae	Jeon S, Kim J	10.1016/j.febslet.2010.10.019
15523097	U3 snoRNP and Rrp5p associate independently with Saccharomyces cerevisiae 35Spre-rRNA, but Rrp5p is essential for association of Rok1p	Vos HR, Bax R, ..., Raue HA	10.1093/nar/gkh904
15358132	Posttranscriptional regulation of the karyogamy gene by Kem1p/Xrn1pexoribonuclease and Rok1p RNA helicase of Saccharomyces cerevisiae	Kim J, Jeon S, Yang YS, Kim J	10.1016/j.bbrc.2004.07.065