Product Description: citrate synthase, mitochondrial, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.3.3.1 (Citrate (Si)-synthase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005759 | mitochondrial matrix |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0004108 | citrate (Si)-synthase activity |
| Process | GO:0046356 | acetyl-CoA catabolic process |
| Process | GO:0005975 | carbohydrate metabolic process |
| Process | GO:0006101 | citrate metabolic process |
| Process | GO:0006099 | tricarboxylic acid cycle |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.39 | 0.83 |
| Trophozoite | expressed | 0.28 | 0.83 |
| Schizont | expressed | 0.28 | 0.81 |
| Gametocyte | expressed | 0.26 | 0.85 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1022500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.91 | #Insertions: 3
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_0506700)
Modification: Tagged | RMgm-4634
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I6U7
"Best" AlphaFill ligand hit: OAA (oxaloacetate ion, Local RMSD=0.09) with 6BOO (Global RMSD=3.49)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.3.1 | citrate (Si)-synthase | ATPSDSNADHHgCl2CuSO4citrateMaleateOuabainD-serineDL-malatepropionateMaleic acid... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P08679 | Citrate synthase, peroxisomal [1-24] | Saccharomyces cerevisiae | HOGENOM, OrthoDB, OrthoMCL | MLS000517255MLS000534613MLS000595372MLS000568342... |
Ortholog Group (OrthoMCL): OG6_101425
Most Similar Human Ortholog: A0A0C4DGI3
TM-align score: 0.98 | RMSD: 1.25
Seq Identity: 0.43 | Length: 400 / 563
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000062485 | citrate synthase |
Protein Length: 563 | Molecular Weight (kDa): 65.041
UniProt ID(s): Q8I6U7
PDB ID(s): None
Isoelectric Point: 9.3
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002020 | Citrate synthase |
| InterPro | IPR019810 | Citrate synthase active site |
| InterPro | IPR036969 | Citrate synthase superfamily |
| PFam | PF00285 | Citrate synthase |
| Superfamily | SSF48256 | Citrate synthase superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 19 | 7 | 33 |
| disruptive | 4 | 13 | 14 | 37 |
| missense | 4 | 13 | 14 | 35 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 47 | 9 | 18 |
| disruptive | 8 | 45 | 17 | 51 |
| missense | 5 | 42 | 16 | 36 |
PlasmoDB Total SNPs: 106
Non-coding: 88 | Synonymous: 16 | Nonsynonymous: 2 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 15279947 | Localization of organellar proteins in Plasmodium falciparum using a novel set oftransfection vectors and a new immunofluorescence fixation method. | Tonkin CJ, van Dooren GG, ..., McFadden GI | 10.1016/j.molbiopara.2004.05.009 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |