Genome location: Pf3D7_10_v3:1,051,357..1,055,540(+)
Genome classification: Core
Product Description: glutamine--fructose-6-phosphate aminotransferase [isomerizing], putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.6.1.16 (Glutamine--fructose-6-phosphate transaminase (isomerizing))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Function | GO:0004360 | glutamine-fructose-6-phosphate transaminase (isomerizing) activity |
| Process | GO:0006047 | UDP-N-acetylglucosamine metabolic process |
| Process | GO:0006002 | fructose 6-phosphate metabolic process |
| Process | GO:0006041 | glucosamine metabolic process |
| Process | GO:0006487 | protein N-linked glycosylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.20 | 0.90 |
| Trophozoite | expressed | 0.37 | 0.78 |
| Schizont | expressed | 0.40 | 0.75 |
| Gametocyte | expressed | 0.33 | 0.80 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1025100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.127 | MFS: -3.187 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0509300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0509300)
Modification: Disrupted | RMgm-4611
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJF3
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.01) with 1AO0 (Global RMSD=3.07)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.6.1.16 | glutamine-fructose-6-phosphate transaminase (isomerizing) | GlyoxalluteolincatechinamitroleazaserineCongo redalbizziinAaptaminenaringeninanticapsinUDP-glucoseiodoacetate... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P17169 | Glutamine--fructose-6-phosphate aminotransferase [isomerizing] | OMA, OrthoMCL | 6-Diazo-5-oxo-L-norleucylglycineN3-(4-methoxyfumaroyl)-L-2,3-diaminopropanoic acid | |
| Q06210 | Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 | Homo sapiens | OMA, OrthoDB | CHEMBL1911368CHEMBL1911368CHEMBL1911372CHEMBL1911373 |
Ortholog Group (OrthoMCL): OG6_100463
Most Similar Human Ortholog: A0A6I8PRN4
TM-align score: 0.85 | RMSD: 2.65
Seq Identity: 0.33 | Length: 550 / 829
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000131459 | glutamine-fructose-6-phosphate transaminase 2 |
| ENSG00000198380 | glutamine--fructose-6-phosphate transaminase 1 |
Protein Length: 829 | Molecular Weight (kDa): 93.659
UniProt ID(s): Q8IJF3
PDB ID(s): None
Isoelectric Point: 9.13
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001347 | Sugar isomerase (SIS) |
| InterPro | IPR005855 | Glucosamine-fructose-6-phosphate aminotransferase, isomerising |
| InterPro | IPR029055 | Nucleophile aminohydrolases, N-terminal |
| InterPro | IPR035466 | GlmS/AgaS, SIS domain 1 |
| InterPro | IPR035490 | GlmS/FrlB, SIS domain 2 |
| PFam | PF01380 | Sugar isomerase (SIS) |
| PFam | PF13522 | |
| Superfamily | SSF53697 | Nucleophile aminohydrolases, N-terminal |
| Superfamily | SSF56235 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 43 | 21 | 49 |
| disruptive | 12 | 45 | 25 | 65 |
| missense | 8 | 43 | 22 | 51 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 87 | 29 | 49 |
| disruptive | 23 | 107 | 56 | 142 |
| missense | 16 | 77 | 48 | 93 |
PlasmoDB Total SNPs: 136
Non-coding: 103 | Synonymous: 23 | Nonsynonymous: 10 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 35215131 | Sheltered in Stromal Tissue Cells, Trypanosoma cruzi Orchestrates InflammatoryNeovascularization via Activation of the Mast Cell Chymase Pathway | Vellasco L, Svensjo E, ..., Scharfstein J | 10.3390/pathogens11020187 |