Genome location: Pf3D7_10_v3:1,125,283..1,126,167(+)
Genome classification: Core
Product Description: biotin--protein ligase 1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.3.1.181 (Lipoyl(octanoyl) transferase);6.3.4.15 (Biotin--[biotin carboxyl-carrier protein] ligase);6.4.1.2 (Acetyl-CoA carboxylase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Function | GO:0004077 | biotin-[acetyl-CoA-carboxylase] ligase activity |
| Process | GO:0042966 | biotin carboxyl carrier protein biosynthetic process |
| Process | GO:0009305 | protein biotinylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | no data | N/A | N/A |
| Ring | no data | 0.00 | 1.00 |
| Trophozoite | no data | 0.01 | 1.00 |
| Schizont | no data | 0.00 | 1.00 |
| Gametocyte | no data | 0.05 | 0.97 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1026900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.996 | MFS: -2.487 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1323600)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0511000)
Modification: Disrupted | RMgm-4603
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6S3D7
"Best" AlphaFill ligand hit: POP (pyrophosphate 2-, Local RMSD=0.10) with 2DKG (Global RMSD=8.18)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.1.181 | lipoyl(octanoyl) transferase | octanoyl-CoA |
| 6.3.4.15 | biotin-[biotin carboxyl-carrier protein] ligase | ATPMgAMPglyceroldiphosphateGLNDIFEAQKIEWHbiotinyl-5'-AMPbiotinol-5'-AMPbiotin acetylene3-(2-oxo-1,3-benzoxazol-3(2H)-yl)-N-phenylpropanamideN-(2-methylphenyl)-2-(2-oxo-1,3-benzoxazol-3(2H)-yl)acetamide2'-amino-5'-[N-(D-biotinoyl)sulfamoyl]amino-3',5'-dideoxyadenosine(2'R,6'S)-9-[6'-([[N-(D-biotinoyl)sulfamoyl]oxy]methyl)morpholin-2'-yl]adenine... |
| 6.4.1.2 | acetyl-CoA carboxylase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100546
Most Similar Human Ortholog: P50747
TM-align score: 0.77 | RMSD: 2.58
Seq Identity: 0.18 | Length: 254 / 294
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000159267 | holocarboxylase synthetase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 9 | 4 | 28 |
| disruptive | 4 | 12 | 13 | 29 |
| missense | 3 | 12 | 13 | 28 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 22 | 20 | 21 |
| disruptive | 7 | 34 | 27 | 64 |
| missense | 6 | 29 | 24 | 48 |
PlasmoDB Total SNPs: 20
Non-coding: 0 | Synonymous: 13 | Nonsynonymous: 7 | Stop Codon: 0
Protein Length: 294 | Molecular Weight (kDa): 34.805
UniProt ID(s): C6S3D7
PDB ID(s): None
Isoelectric Point: 6.71
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004143 | Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domain |
| InterPro | IPR004408 | Biotin--acetyl-CoA-carboxylase ligase |
| PFam | PF03099 | Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domain |
| Superfamily | SSF55681 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 24583112 | Apicoplast acetyl Co-A carboxylase of the human malaria parasite is not targetedby cyclohexanedione herbicides. | Goodman CD, Mollard V, ..., McFadden GI | 10.1016/j.ijpara.2014.01.007 |
| 29483266 | Host biotin is required for liver stage development in malaria parasites. | Dellibovi-Ragheb TA, Jhun H, ..., Prigge ST | 10.1073/pnas.1800717115 |
| 31094037 | Development of a conditional localization approach to control apicoplast proteintrafficking in malaria parasites. | Roberts AD, Nair SC, Guerra AJ, Prigge ST | 10.1111/tra.12656 |
| 26829225 | Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 A resolution | Nguyen THD, Galej WP, ..., Nagai K | 10.1038/nature16940 |
| 34542280 | Random Base Editing for Genome Evolution in Saccharomyces cerevisiae | Pan Y, Xia S, ..., Lian J | 10.1021/acssynbio.1c00217 |
| 20024080 | Metabolic profiling of the fission yeast S. pombe: quantification of compoundsunder different temperatures and genetic perturbation | Pluskal T, Nakamura T, Villar-Briones A, Yanagida M | 10.1039/b908784b |