Genome location: Pf3D7_10_v3:1,145,400..1,147,484(-)
Genome classification: Core
Product Description: DNA-directed RNA polymerase II subunit RPB7, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0000932 | P-body |
| Component | GO:0005665 | RNA polymerase II, core complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0003968 | RNA-directed 5'-3' RNA polymerase activity |
| Function | GO:0003697 | single-stranded DNA binding |
| Function | GO:0003727 | single-stranded RNA binding |
| Function | GO:0031369 | translation initiation factor binding |
| Process | GO:0000291 | nuclear-transcribed mRNA catabolic process, exonucleolytic |
| Process | GO:0060213 | positive regulation of nuclear-transcribed mRNA poly(A) tail shortening |
| Process | GO:0045948 | positive regulation of translational initiation |
| Process | GO:0006367 | transcription initiation from RNA polymerase II promoter |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.09 | 0.95 |
| Trophozoite | expressed | 0.06 | 0.96 |
| Schizont | expressed | 0.03 | 0.98 |
| Gametocyte | expressed | 0.04 | 0.98 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1027400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.145 | MFS: -2.646 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0511500)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJC9
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.6 | DNA-directed RNA polymerase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102167
Most Similar Human Ortholog: P62487
TM-align score: 0.96 | RMSD: 0.91
Seq Identity: 0.39 | Length: 170 / 177
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000168002 | RNA polymerase II subunit G |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 147
Non-coding: 141 | Synonymous: 5 | Nonsynonymous: 1 | Stop Codon: 0
Protein Length: 177 | Molecular Weight (kDa): 20.341
UniProt ID(s): Q8IJC9
PDB ID(s): None
Isoelectric Point: 7.65
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003029 | S1 domain |
| InterPro | IPR005576 | RNA polymerase Rpb7-like , N-terminal |
| InterPro | IPR012340 | Nucleic acid-binding, OB-fold |
| InterPro | IPR036898 | RNA polymerase Rpb7-like, N-terminal domain superfamily |
| PFam | PF00575 | S1 domain |
| PFam | PF03876 | RNA polymerase Rpb7-like , N-terminal |
| Superfamily | SSF50249 | Nucleic acid-binding, OB-fold |
| Superfamily | SSF88798 | RNA polymerase Rpb7-like, N-terminal domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30395243 | Genome-wide transcriptional response to altered levels of the Rpb7 subunit of RNApolymerase II identifies its role in DNA damage response in Schizosaccharomycespombe | Kumar D, Sharma N | 10.1093/femsyr/foy118 |
| 25530312 | Modulating the level of the Rpb7 subunit of RNA polymerase II affects cellseparation in Schizosaccharomyces pombe | Kumar D, Sharma N | 10.1016/j.resmic.2014.12.002 |
| 30862564 | A comparative study of the proteome regulated by the Rpb4 and Rpb7 subunits ofRNA polymerase II in fission yeast | Kumar D, Varshney S, Sengupta S, Sharma N | 10.1016/j.jprot.2019.03.007 |
| 35905859 | Rpb4/7, a key element of RNA pol II to coordinate mRNA synthesis in the nucleuswith cytoplasmic functions in Saccharomyces cerevisiae | Garrido-Godino AI, Martin-Exposito M, ..., Navarro F | 10.1016/j.bbagrm.2022.194846 |
| 30359412 | Dissociation of Rpb4 from RNA polymerase II is important for yeast functionality | Duek L, Barkai O, ..., Choder M | 10.1371/journal.pone.0206161 |
| 28515784 | Improving Saccharomyces cerevisiae ethanol production and tolerance via RNApolymerase II subunit Rpb7 | Qiu Z, Jiang R | 10.1186/s13068-017-0806-0 |
| 35051353 | Structural visualization of de novo transcription initiation by Saccharomycescerevisiae RNA polymerase II | Yang C, Fujiwara R, ..., Murakami K | 10.1016/j.molcel.2021.12.020 |