PF3D7_1027600 (SPO11-2)

Genome location: Pf3D7_10_v3:1,148,267..1,150,490(-)

Genome classification: Core

Function and Localization

Product Description: meiotic recombination protein SPO11-2, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 5.99.1.3 (Transferred entry: 5.6.2.2)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0000228	nuclear chromosome
Function	GO:0003677	DNA binding
Function	GO:0016889	endodeoxyribonuclease activity, producing 3'-phosphomonoesters
Process	GO:0042138	meiotic DNA double-strand break formation
Process	GO:0000706	meiotic DNA double-strand break processing
Process	GO:0007131	reciprocal meiotic recombination

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	no data	N/A	N/A
Ring	no data	0.10	0.95
Trophozoite	no data	0.08	0.95
Schizont	no data	0.07	0.95
Gametocyte	no data	0.40	0.78

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1027600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.124 | MFS: -3.101 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1432700)

Relative Growth Rate: 0.99 ± 0.16
Confidence: 5.04

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6S3D8

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
5.99.1.3	DNA topoisomerase (ATP-hydrolysing)	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101269

Most Similar Human Ortholog: Q9Y5K1

TM-align score: 0.77 | RMSD: 3.56

Seq Identity: 0.25 | Length: 305 / 336

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000054796	SPO11 initiator of meiotic double stranded breaks

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	19	13	23
disruptive	8	35	12	59
missense	8	35	12	59

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	3	34	20	23
disruptive	11	73	42	98
missense	11	65	39	77

PlasmoDB Total SNPs: 179

Non-coding: 161 | Synonymous: 9 | Nonsynonymous: 9 | Stop Codon: 0

Protein Information

Protein Length: 336 | Molecular Weight (kDa): 39.605

UniProt ID(s): C6S3D8

PDB ID(s): None

Isoelectric Point: 9.07

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002815	Spo11/DNA topoisomerase VI subunit A
InterPro	IPR013049	Spo11/DNA topoisomerase VI, subunit A, N-terminal
InterPro	IPR034136	Topoisomerase 6 subunit A/Spo11, TOPRIM domain
InterPro	IPR036078	Spo11/DNA topoisomerase VI subunit A superfamily
PFam	PF04406	Spo11/DNA topoisomerase VI, subunit A, N-terminal
Superfamily	SSF56726	Spo11/DNA topoisomerase VI subunit A superfamily