PF3D7_1027900 (DHHC10)

Genome location: Pf3D7_10_v3:1,156,648..1,158,980(+)

Genome classification: Core

Function and Localization

Product Description: palmitoyltransferase DHHC10, putative

SignalP Peptide: N/A

# Transmembrane Domains: 4

EC Numbers: 2.3.1.- (Transferring groups other than amino-acyl groups.);2.3.1.225 (Protein S-acyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005794	Golgi apparatus
Component	GO:0044312	crystalloid
Component	GO:0005783	endoplasmic reticulum
Function	GO:0016409	palmitoyltransferase activity
Function	GO:0019706	protein-cysteine S-palmitoyltransferase activity
Process	GO:0018230	peptidyl-L-cysteine S-palmitoylation
Process	GO:0018345	protein palmitoylation
Process	GO:0006612	protein targeting to membrane

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	possibly expressed	0.01	0.99
Trophozoite	possibly expressed	0.03	0.98
Schizont	possibly expressed	0.01	0.99
Gametocyte	expressed	0.58	0.75

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1027900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.999 | MFS: -0.532 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Not tested (Pb ortholog: PY17X_0513100)

Modification: Tagged | RMgm-4503

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A143ZYC1

"Best" AlphaFill ligand hit: DYD ((2s,5s)-hexane-2,5-diol, Local RMSD=0.37) with 6BMM (Global RMSD=2.29)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.3.1.-	Ex: [myelin-proteolipid] O-palmitoyltransferase	CoAKClArgATPADPEDTAMgCl2CaCl2MnCl2AgNO3CoCl2FeSO4...
2.3.1.225	protein S-acyltransferase	stearoyl-CoAmyristoyl-CoA2-Bromopalmitate2-bromo-palmitateacyl-CoA binding protein

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
Q9UIJ5	Palmitoyltransferase ZDHHC2		OrthoMCL BLAST	CHEMBL118417CHEMBL5201039CHEMBL5199102CHEMBL5186633

Orthology Information

Ortholog Group (OrthoMCL): OG6_119670

No human ortholog(s)

Protein Information

Protein Length: 285 | Molecular Weight (kDa): 33.831

UniProt ID(s): A0A143ZYC1

PDB ID(s): None

Isoelectric Point: 8

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001594	Palmitoyltransferase, DHHC domain
PFam	PF01529	Palmitoyltransferase, DHHC domain
Superfamily	N/A	N/A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	14	9	15
disruptive	0	5	8	14
missense	0	5	8	14

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	32	12	26
disruptive	1	23	15	54
missense	1	16	13	36

PlasmoDB Total SNPs: 201

Non-coding: 187 | Synonymous: 11 | Nonsynonymous: 2 | Stop Codon: 1

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
12368868	Sequence of Plasmodium falciparum chromosomes 2, 10, 11 and 14.	Gardner MJ, Shallom SJ, ..., Fraser CM	10.1038/nature01094
27303037	Maternally supplied S-acyl-transferase is required for crystalloid organelleformation and transmission of the malaria parasite.	Santos JM, Duarte N, ..., Mair GR	https://pubmed.ncbi.nlm.nih.gov/27303037/