PF3D7_1028000

Genome location: Pf3D7_10_v3:1,159,580..1,163,736(+)

Genome classification: Core

Function and Localization

Product Description: methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.61 (tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Function	GO:0016706	2-oxoglutarate-dependent dioxygenase activity
Function	GO:0016300	tRNA (uracil) methyltransferase activity
Function	GO:0000049	tRNA binding
Process	GO:0002098	tRNA wobble uridine modification

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.29	0.86
Trophozoite	expressed	0.36	0.80
Schizont	not expressed	0.05	0.97
Gametocyte	not expressed	0.38	0.79

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1028000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.846 | #Insertions: 4

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0512100)

Relative Growth Rate: 0.38 ± 0.10
Confidence: 5.96

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0512100)

Modification: Disrupted | RMgm-1988

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJC4

"Best" AlphaFill ligand hit: AKG (2-oxoglutaric acid, Local RMSD=0.96) with 3THT (Global RMSD=2.03)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.61	tRNA 5-(aminomethyl)-2-thiouridylate-methyltransferase	p-chloromercuribenzoate

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100549

Most Similar Human Ortholog: E9PK20

TM-align score: 0.55 | RMSD: 2.38

Seq Identity: 0.33 | Length: 48 / 593

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000137760	alkB homolog 8, tRNA methyltransferase
ENSG00000250305	tRNA methyltransferase 9B (putative)
ENSG00000284872	tRNA methyltransferase 9B (putative)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	25	20	47
disruptive	6	42	25	73
missense	5	37	23	68

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	56	32	45
disruptive	12	100	68	120
missense	6	86	56	84

PlasmoDB Total SNPs: 318

Non-coding: 285 | Synonymous: 18 | Nonsynonymous: 15 | Stop Codon: 0

Protein Information

Protein Length: 593 | Molecular Weight (kDa): 70.567

UniProt ID(s): Q8IJC4

PDB ID(s): None

Isoelectric Point: 7.92

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR013216	Methyltransferase type 11
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
InterPro	IPR032863	Alkylated DNA repair protein alkB homologue 8
PFam	PF08241	Methyltransferase type 11
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase