Genome location: Pf3D7_10_v3:1,163,786..1,165,615(+)
Genome classification: Core
Product Description: protoporphyrinogen oxidase
SignalP Peptide: N/A
# Transmembrane Domains: 2
EC Numbers: 1.3.3.4 (Protoporphyrinogen oxidase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0031304 | intrinsic component of mitochondrial inner membrane |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0016491 | oxidoreductase activity |
| Function | GO:0004729 | oxygen-dependent protoporphyrinogen oxidase activity |
| Process | GO:0006783 | heme biosynthetic process |
| Process | GO:0019353 | protoporphyrinogen IX biosynthetic process from glutamate |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.06 | 0.97 |
| Trophozoite | not expressed | 0.06 | 0.96 |
| Schizont | not expressed | 0.05 | 0.97 |
| Gametocyte | not expressed | 0.19 | 0.89 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1028100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.495 | #Insertions: 3
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0512200)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0512200)
Modification: Disrupted | RMgm-1989
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJC3
"Best" AlphaFill ligand hit: FAD (flavin-adenine dinucleotide, Local RMSD=0.79) with 3I6D (Global RMSD=2.57)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.3.3.4 | protoporphyrinogen oxidase | BRENDA SOAP query unsuccessful |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P32397 | Coproporphyrinogen III oxidase | Bacillus subtilis | OrthoMCL | CHEMBL576568CHEMBL577803CHEMBL573126CHEMBL578226... |
| O24163 | Protoporphyrinogen oxidase, chloroplastic | OrthoMCL BLAST | CHEMBL2286221CHEMBL2286218CHEMBL2286235CHEMBL2286222... | |
| Q9M629 | Protoporphyrinogen oxidase | OrthoMCL BLAST | CHEMBL2253320CHEMBL2253455CHEMBL2253454CHEMBL2253323... |
Ortholog Group (OrthoMCL): OG6_101704
Most Similar Human Ortholog: A0A494C146
TM-align score: 0.85 | RMSD: 2.89
Seq Identity: 0.17 | Length: 405 / 609
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000143224 | protoporphyrinogen oxidase |
Protein Length: 609 | Molecular Weight (kDa): 72.232
PDB ID(s): None
Isoelectric Point: 9.32
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002937 | Amine oxidase |
| InterPro | IPR036188 | FAD/NAD(P)-binding domain superfamily |
| PFam | PF01593 | Amine oxidase |
| Superfamily | SSF51905 | FAD/NAD(P)-binding domain superfamily |
| Superfamily | SSF54373 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 27 | 18 | 45 |
| disruptive | 9 | 53 | 43 | 78 |
| missense | 9 | 51 | 39 | 71 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 59 | 36 | 51 |
| disruptive | 27 | 104 | 60 | 128 |
| missense | 19 | 87 | 55 | 96 |
PlasmoDB Total SNPs: 33
Non-coding: 0 | Synonymous: 22 | Nonsynonymous: 11 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 20603160 | Protoporphyrinogen IX oxidase from Plasmodium falciparum is anaerobic and islocalized to the mitochondrion. | Nagaraj VA, Arumugam R, ..., Padmanaban G | 10.1016/j.molbiopara.2010.06.012 |