Genome location: Pf3D7_10_v3:1,179,469..1,183,381(+)
Genome classification: Core
Product Description: merozoite TRAP-like protein
SignalP Peptide: MKKTILNLYLINILFALSDVKG
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0009986 | cell surface |
| Component | GO:0020009 | microneme |
| Function | GO:0046789 | host cell surface receptor binding |
| Process | GO:0044409 | entry into host |
| Process | GO:0035891 | exit from host cell |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.48 | 0.85 |
| Trophozoite | expressed | 0.25 | 0.84 |
| Schizont | expressed | 0.59 | 0.76 |
| Gametocyte | expressed | 0.25 | 0.85 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1028700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 1 | MFS: -1.824 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0512800)
Modification: Disrupted | RMgm-4519
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJB7
"Best" AlphaFill ligand hit: FAD (flavin-adenine dinucleotide, Local RMSD=0.30) with 2OK7 (Global RMSD=28.95)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 25 | 18 | 25 |
| disruptive | 28 | 82 | 37 | 106 |
| missense | 20 | 63 | 30 | 82 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 49 | 20 | 31 |
| disruptive | 51 | 178 | 73 | 176 |
| missense | 41 | 136 | 51 | 101 |
PlasmoDB Total SNPs: 241
Non-coding: 180 | Synonymous: 25 | Nonsynonymous: 36 | Stop Codon: 0
Protein Length: 498 | Molecular Weight (kDa): 58.085
UniProt ID(s): Q8IJB7
PDB ID(s): None
Isoelectric Point: 4.44
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | N/A | N/A |
| PFam | N/A | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 16321976 | A conserved molecular motor drives cell invasion and gliding motility acrossmalaria life cycle stages and other apicomplexan parasites. | Baum J, Richard D, ..., Cowman AF | 10.1074/jbc.M509807200 |
| 22467743 | Analysis of the conformation and function of the Plasmodium falciparum merozoiteproteins MTRAP and PTRAMP. | Uchime O, Herrera R, ..., Narum DL | 10.1128/EC.00039-12 |
| 23166499 | Semaphorin-7A is an erythrocyte receptor for P. falciparum merozoite-specificTRAP homolog, MTRAP. | Bartholdson SJ, Bustamante LY, ..., Wright GJ | 10.1371/journal.ppat.1003031 |
| 27832590 | Plasmodium Merozoite TRAP Family Protein Is Essential for Vacuole MembraneDisruption and Gamete Egress from Erythrocytes. | Bargieri DY, Thiberge S, ..., Menard R | 10.1016/j.chom.2016.10.015 |
| 35906227 | Tryptophan C-mannosylation is critical for Plasmodium falciparum transmission | Lopaticki S, McConville R, ..., Boddey JA | 10.1038/s41467-022-32076-8 |