Genome location: Pf3D7_10_v3:1,204,815..1,207,912(+)
Genome classification: Core
Product Description: adenosine deaminase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.5.4.4 (Adenosine deaminase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005829 | cytosol |
| Component | GO:0009897 | external side of plasma membrane |
| Function | GO:0004000 | adenosine deaminase activity |
| Process | GO:0006154 | adenosine catabolic process |
| Process | GO:0043103 | hypoxanthine salvage |
| Process | GO:0046103 | inosine biosynthetic process |
| Process | GO:0060169 | negative regulation of adenosine receptor signaling pathway |
| Process | GO:0042493 | response to drug |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.26 | 0.88 |
| Trophozoite | expressed | 0.66 | 0.65 |
| Schizont | expressed | 0.07 | 0.95 |
| Gametocyte | expressed | 0.12 | 0.94 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1029600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.121 | MFS: -2.967 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0513600)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0513600)
Modification: Disrupted | RMgm-1996
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJA9
"Best" AlphaFill ligand hit: NHE (2-[n-cyclohexylamino]ethane sulfonic acid, Local RMSD=0.22) with 2QVN (Global RMSD=1.26)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.5.4.4 | adenosine deaminase | ATPAMPIgGEDTANH4+DEPCHgCl2Na2SO4purineethanoladenineInosine... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q86GS5 | Adenosine deaminase | OMA, OrthoMCL, OrthoMCL BLAST | DeoxycoformycinCHEMBL1234234COFORMYCINDeoxycoformycin... | |
| P00813 | Adenosine deaminase | Homo sapiens | OMA, OrthoMCL BLAST | FR221647CHEMBL93647FR230513FR235999... |
| P03958 | Adenosine deaminase | Mus musculus | OMA, OrthoMCL BLAST | CHEBI:46938CHEMBL49935CHEMBL93560 |
| A0A1S5RQP7 | Adenosine deaminase | OrthoMCL BLAST | Pentostatin (PTN)Pentostatin (PTN) | |
| P56658 | Adenosine deaminase | Bos taurus | OrthoMCL BLAST | pyrazolo[3,4-d]pyrimidin-4-one, 13cpyrazolo[3,4-d]pyrimidin-4-one, 13dpyrazolo[3,4-d]pyrimidin-4-one, 14apyrazolo[3,4-d]pyrimidin-4-one, 14c... |
Ortholog Group (OrthoMCL): OG6_103627
Most Similar Human Ortholog: P00813
TM-align score: 0.84 | RMSD: 2.11
Seq Identity: 0.25 | Length: 329 / 367
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000196839 | adenosine deaminase |
Protein Length: 367 | Molecular Weight (kDa): 42.466
PDB ID(s): 6II7
Isoelectric Point: 5.43
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001365 | Adenosine/AMP deaminase domain |
| InterPro | IPR006330 | Adenosine/adenine deaminase |
| InterPro | IPR006650 | Adenosine/AMP deaminase active site |
| InterPro | IPR032466 | Metal-dependent hydrolase |
| PFam | PF00962 | Adenosine/AMP deaminase domain |
| Superfamily | SSF51556 | Metal-dependent hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 22 | 10 | 22 |
| disruptive | 6 | 17 | 11 | 32 |
| missense | 6 | 17 | 11 | 27 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 38 | 18 | 20 |
| disruptive | 11 | 48 | 18 | 62 |
| missense | 10 | 37 | 16 | 42 |
PlasmoDB Total SNPs: 192
Non-coding: 178 | Synonymous: 8 | Nonsynonymous: 6 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 15576366 | Targeting a novel Plasmodium falciparum purine recycling pathway with specificimmucillins. | Ting LM, Shi W, ..., Kim K | 10.1074/jbc.M412693200 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 22446744 | Mapping the genome of Plasmodium falciparum on the drug-like chemical spacereveals novel anti-malarial targets and potential drug leads. | Jensen K, Plichta D, Panagiotou G, Kouskoumvekaki I | 10.1039/c2mb00008c |
| 31002765 | Crystal structure of Plasmodium falciparum adenosine deaminase reveals a novelbinding pocket for inosine. | Jaruwat A, Riangrungroj P, ..., Chitnumsub P | 10.1016/j.abb.2019.04.002 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 38400149 | Persistence of Anti-SE36 Antibodies Induced by the Malaria Vaccine CandidateBK-SE36/CpG in 5-10-Year-Old Burkinabe Children Naturally Exposed to Malaria | Nebie I, Palacpac NMQ, ..., Sirima SB | 10.3390/vaccines12020166 |