Genome location: Pf3D7_10_v3:1,223,717..1,229,643(-)
Genome classification: Core
Product Description: pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0071013 | catalytic step 2 spliceosome |
| Function | GO:0003723 | RNA binding |
| Function | GO:0003724 | RNA helicase activity |
| Process | GO:0008380 | RNA splicing |
| Process | GO:0000390 | spliceosomal complex disassembly |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.66 | 0.69 |
| Trophozoite | expressed | 0.86 | 0.54 |
| Schizont | expressed | 0.32 | 0.80 |
| Gametocyte | expressed | 0.78 | 0.58 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1030100
Resistome Missense Mutations: E253D
Resistome Compounds with Missense Mutations: MMV024101
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.138 | MFS: -2.78 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1446300)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJA4
"Best" AlphaFill ligand hit: MPO (3[n-morpholino]propane sulfonic acid, Local RMSD=0.18) with 6ZM2 (Global RMSD=2.72)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.13 | RNA helicase | ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100085
Most Similar Human Ortholog: A0A140T8Y5
TM-align score: 0.94 | RMSD: 2.03
Seq Identity: 0.47 | Length: 546 / 1290
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000005100 | DEAH-box helicase 33 |
| ENSG00000067596 | DEAH-box helicase 8 |
| ENSG00000101452 | DEAH-box helicase 35 |
| ENSG00000109606 | DEAH-box helicase 15 |
| ENSG00000140829 | DEAH-box helicase 38 |
| ENSG00000204560 | DEAH-box helicase 16 |
| ENSG00000206486 | DEAH-box helicase 16 |
| ENSG00000226171 | DEAH-box helicase 16 |
| ENSG00000227222 | DEAH-box helicase 16 |
| ENSG00000231377 | DEAH-box helicase 16 |
| ENSG00000233049 | DEAH-box helicase 16 |
| ENSG00000233418 | DEAH-box helicase 16 |
| ENSG00000233561 | DEAH-box helicase 16 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 61 | 37 | 70 |
| disruptive | 23 | 59 | 44 | 117 |
| missense | 15 | 50 | 37 | 98 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 21 | 125 | 68 | 83 |
| disruptive | 38 | 162 | 96 | 236 |
| missense | 21 | 118 | 78 | 181 |
PlasmoDB Total SNPs: 238
Non-coding: 130 | Synonymous: 34 | Nonsynonymous: 74 | Stop Codon: 0
Protein Length: 1290 | Molecular Weight (kDa): 149.368
UniProt ID(s): Q8IJA4
PDB ID(s): None
Isoelectric Point: 8.78
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR002464 | DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site |
| InterPro | IPR003029 | S1 domain |
| InterPro | IPR007502 | Helicase-associated domain |
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR011709 | Domain of unknown function DUF1605 |
| InterPro | IPR012340 | Nucleic acid-binding, OB-fold |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR022967 | RNA-binding domain, S1 |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| PFam | PF00271 | Helicase, C-terminal |
| PFam | PF00575 | S1 domain |
| PFam | PF04408 | Helicase-associated domain |
| PFam | PF07717 | Domain of unknown function DUF1605 |
| Superfamily | SSF50249 | Nucleic acid-binding, OB-fold |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 37625852 | Biochemical and genetic evidence supports Fyv6 as a second-step splicing factorin Saccharomyces cerevisiae | Lipinski KA, Senn KA, Zeps NJ, Hoskins AA | 10.1261/rna.079607.123 |
| 29146870 | Structure of the yeast spliceosomal postcatalytic P complex | Liu S, Li X, ..., Zhao R | 10.1126/science.aar3462 |
| 29153833 | Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae | Bai R, Yan C, ..., Shi Y | 10.1016/j.cell.2017.10.038 |
| 36778415 | Biochemical and Genetic Evidence Supports Fyv6 as a Second-Step Splicing Factorin Saccharomyces cerevisiae | Lipinski KA, Senn KA, Zeps NJ, Hoskins AA | 10.1101/2023.01.30.526368 |