Genome location: Pf3D7_10_v3:1,243,547..1,245,815(+)
Genome classification: Core
Product Description: 26S proteasome regulatory subunit RPN9, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.25.1 (Proteasome endopeptidase complex)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Component | GO:0005838 | proteasome regulatory particle |
| Component | GO:0008541 | proteasome regulatory particle, lid subcomplex |
| Function | GO:0004175 | endopeptidase activity |
| Function | GO:0005198 | structural molecule activity |
| Process | GO:0043248 | proteasome assembly |
| Process | GO:0006511 | ubiquitin-dependent protein catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.24 | 0.88 |
| Trophozoite | not expressed | 0.80 | 0.56 |
| Schizont | not expressed | 0.40 | 0.75 |
| Gametocyte | not expressed | 0.43 | 0.75 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1030500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.209 | MFS: -3.366 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0514500)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJA0
"Best" AlphaFill ligand hit: UPG (uridine-5'-diphosphate-glucose, Local RMSD=3.47) with 2VKD (Global RMSD=19.61)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.25.1 | proteasome endopeptidase complex | ATPSDSPSIEDTANH4+EGTATLCKALLNNMDANLVSPI 1PA28... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101776
Most Similar Human Ortholog: Q9UNM6
TM-align score: 0.87 | RMSD: 2.82
Seq Identity: 0.23 | Length: 367 / 393
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000185627 | proteasome 26S subunit, non-ATPase 13 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 25 | 7 | 23 |
| disruptive | 3 | 23 | 18 | 37 |
| missense | 3 | 21 | 18 | 31 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 42 | 16 | 21 |
| disruptive | 10 | 54 | 39 | 68 |
| missense | 6 | 46 | 31 | 42 |
PlasmoDB Total SNPs: 62
Non-coding: 48 | Synonymous: 8 | Nonsynonymous: 6 | Stop Codon: 0
Protein Length: 393 | Molecular Weight (kDa): 46.801
UniProt ID(s): Q8IJA0
PDB ID(s): None
Isoelectric Point: 5.93
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000717 | Proteasome component (PCI) domain |
| InterPro | IPR035298 | 26S Proteasome non-ATPase regulatory subunit 13 |
| InterPro | IPR036390 | Winged helix DNA-binding domain superfamily |
| PFam | PF01399 | Proteasome component (PCI) domain |
| Superfamily | SSF46785 | Winged helix DNA-binding domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 23832675 | (1)H, (1)(3)C and (1)(5)N resonance assignments of Rpn9, a regulatory subunit of 26Sproteasome from Saccharomyces cerevisiae | Wu Y, Hu Y, Jin C | 10.1007/s12104-013-9506-2 |
| 17761670 | Isolation of the Schizosaccharomyces pombe proteasome subunit Rpn7 and astructure-function study of the proteasome-COP9-initiation factor domain | Sha Z, Yen HC, ..., Chang EC | 10.1074/jbc.M706276200 |
| 29229809 | Proteasomes tether to two distinct sites at the nuclear pore complex | Albert S, Schaffer M, ..., Engel BD | 10.1073/pnas.1716305114 |
| 12437114 | Reverse genetic analysis of the Caenorhabditis elegans 26S proteasome subunits byRNA interference | Takahashi M, Iwasaki H, Inoue H, Takahashi K | 10.1515/BC.2002.140 |
| 25631053 | Solution structure of yeast Rpn9: insights into proteasome lid assembly | Hu Y, Wu Y, ..., Jin C | 10.1074/jbc.M114.626762 |
| 20471955 | Dissection of the assembly pathway of the proteasome lid in Saccharomycescerevisiae | Fukunaga K, Kudo T, ..., Saeki Y | 10.1016/j.bbrc.2010.05.061 |
| 15533439 | Uch2/Uch37 is the major deubiquitinating enzyme associated with the 26S proteasome in fission yeast | Stone M, Hartmann-Petersen R, ..., Gordon C | 10.1016/j.jmb.2004.09.057 |
| 10490597 | Rpn9 is required for efficient assembly of the yeast 26S proteasome | Takeuchi J, Fujimuro M, ..., Toh-e A | 10.1128/MCB.19.10.6575 |
| 12147266 | Increased degradation of oxidized proteins in yeast defective in 26 S proteasomeassembly | Inai Y, Nishikimi M | 10.1016/s0003-9861(02)00336-3 |
| 11295494 | Genetic dissection of the yeast 26S proteasome: cell cycle defects caused by theDeltarpn9 mutation | Takeuchi J, Toh-e A | 10.1016/s0300-9084(01)01238-x |
| 36060813 | Proteasomal subunit depletions differentially affect germline integrity in C.elegans | Fernando LM, Quesada-Candela C, ..., Allen AK | 10.3389/fcell.2022.901320 |