Genome location: Pf3D7_10_v3:1,245,421..1,247,811(-)
Genome classification: Core
Product Description: tRNA N6-adenosine threonylcarbamoyltransferase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.3.1.234 (N(6)-L-threonylcarbamoyladenine synthase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0000408 | EKC/KEOPS complex |
| Component | GO:0005737 | cytoplasm |
| Function | GO:0005515 | protein binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.41 | 0.80 |
| Trophozoite | expressed | 0.50 | 0.71 |
| Schizont | expressed | 0.11 | 0.93 |
| Gametocyte | expressed | 0.20 | 0.88 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1030600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.912 | MFS: -2.195 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1006500)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJ99
"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.03) with 6Z81 (Global RMSD=2.42)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.1.234 | N6-L-threonylcarbamoyladenine synthase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100288
Most Similar Human Ortholog: Q9NPF4
TM-align score: 0.96 | RMSD: 1.50
Seq Identity: 0.46 | Length: 334 / 598
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000092094 | O-sialoglycoprotein endopeptidase |
| ENSG00000128694 | O-sialoglycoprotein endopeptidase like 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 38 | 18 | 55 |
| disruptive | 8 | 45 | 36 | 106 |
| missense | 5 | 40 | 34 | 95 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 76 | 25 | 46 |
| disruptive | 17 | 126 | 70 | 134 |
| missense | 12 | 102 | 62 | 106 |
PlasmoDB Total SNPs: 123
Non-coding: 73 | Synonymous: 25 | Nonsynonymous: 25 | Stop Codon: 0
Protein Length: 598 | Molecular Weight (kDa): 69.574
UniProt ID(s): Q8IJ99
PDB ID(s): None
Isoelectric Point: 7.59
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000905 | Gcp-like domain |
| InterPro | IPR017861 | Kae1/TsaD family |
| InterPro | IPR034680 | tRNA N6-adenosine threonylcarbamoyltransferase Kae1, archaea and eukaryote |
| InterPro | IPR043129 | ATPase, nucleotide binding domain |
| PFam | PF00814 | Gcp-like domain |
| Superfamily | SSF53067 | ATPase, nucleotide binding domain |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 25204654 | Kinase-associated endopeptidase 1 (Kae1) participates in an atypicalribosome-associated complex in the apicoplast of Plasmodium falciparum. | Mallari JP, Oksman A, Vaupel B, Goldberg DE | 10.1074/jbc.M114.586735 |
| 34033682 | Boosting endoplasmic reticulum folding capacity reduces unfolded protein responseactivation and intracellular accumulation of human kidney anion exchanger 1 inSaccharomyces cerevisiae | Li X, Cordat E, Schmitt MJ, Becker B | 10.1002/yea.3652 |
| 31996424 | Saccharomyces cerevisiae: First Steps to a Suitable Model System To Study theFunction and Intracellular Transport of Human Kidney Anion Exchanger 1 | Sarder HAM, Li X, ..., Becker B | 10.1128/mSphere.00802-19 |
| 31417508 | KAE1 Allelic Variants Affect TORC1 Activation and Fermentation Kinetics inSaccharomyces cerevisiae | Kessi-Perez EI, Salinas F, ..., Martinez C | 10.3389/fmicb.2019.01686 |
| 36416748 | Kae1 of Saccharomyces cerevisiae KEOPS complex possesses ADP/GDP nucleotidaseactivity | Li QX, Liu JC, He MH, Zhou JQ | 10.1042/BCJ20220290 |