PF3D7_1030700

Genome location: Pf3D7_10_v3:1,248,115..1,249,462(+)

Genome classification: Core

Function and Localization

Product Description: RNA methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.34 (tRNA (guanosine(18)-2'-O)-methyltransferase)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.39	0.81
Trophozoite	expressed	0.44	0.75
Schizont	expressed	0.04	0.98
Gametocyte	expressed	0.16	0.90

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1030700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.126 | MFS: -2.759 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJ98

"Best" AlphaFill ligand hit: SAM (s-adenosylmethionine, Local RMSD=0.71) with 1V2X (Global RMSD=1.96)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.34	tRNA (guanosine18-2'-O)-methyltransferase	PolyamineDELTAD-armS-adenosyl-L-homocysteine

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_111165

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	14	3	15
disruptive	2	26	13	26
missense	2	25	12	23

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	4	20	4	21
disruptive	7	48	25	58
missense	4	41	22	45

PlasmoDB Total SNPs: 99

Non-coding: 85 | Synonymous: 9 | Nonsynonymous: 5 | Stop Codon: 0

Protein Information

Protein Length: 257 | Molecular Weight (kDa): 29.978

UniProt ID(s): Q8IJ98

PDB ID(s): None

Isoelectric Point: 8.98

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001537	tRNA/rRNA methyltransferase, SpoU type
InterPro	IPR029028	Alpha/beta knot methyltransferases
PFam	PF00588	tRNA/rRNA methyltransferase, SpoU type
Superfamily	SSF75217	Alpha/beta knot methyltransferases