PF3D7_1030900 (P28)

Genome location: Pf3D7_10_v3:1,251,151..1,251,807(-)

Genome classification: Core

Function and Localization

Product Description: ookinete surface protein P28

SignalP Peptide: MNTYFKVLLFLFIQLYITLNKA

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.00	1.00
Trophozoite	not expressed	0.00	1.00
Schizont	possibly expressed	0.01	0.99
Gametocyte	expressed	0.13	0.92

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1030900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -2.91 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0515000)

Relative Growth Rate: 1.00 ± 0.01
Confidence: 10.00

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PY17X_0515900)

Modification: Tagged | RMgm-4071

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJ96

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104197

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	17	3	18
disruptive	9	19	9	26
missense	9	19	9	24

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	1	30	5	19
disruptive	11	39	24	49
missense	11	36	21	47

PlasmoDB Total SNPs: 12

Non-coding: 0 | Synonymous: 4 | Nonsynonymous: 8 | Stop Codon: 0

Protein Information

Protein Length: 218 | Molecular Weight (kDa): 24.266

UniProt ID(s): Q6LEB4, Q8IJ96

PDB ID(s): None

Isoelectric Point: 6.2

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000742	EGF-like domain
InterPro	IPR010423	Ookinete surface antigen, EGF domain
PFam	PF06247	Ookinete surface antigen, EGF domain
Superfamily	N/A	N/A