Genome location: Pf3D7_10_v3:1,253,373..1,254,412(-)
Genome classification: Core
Product Description: ookinete surface protein P25
SignalP Peptide: MNKLYSLFLFLFIQLSIKYNNA
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0046658 | anchored component of plasma membrane |
| Component | GO:0005886 | plasma membrane |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.02 | 0.99 |
| Trophozoite | not expressed | 0.01 | 0.99 |
| Schizont | not expressed | 0.02 | 0.99 |
| Gametocyte | expressed | 1.60 | 0.59 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1031000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: 0.688 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0515000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0515000)
Modification: Disrupted | RMgm-2004
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q7KQL2
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 6 | 7 | 17 |
| disruptive | 3 | 4 | 3 | 15 |
| missense | 3 | 4 | 3 | 13 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 14 | 8 | 27 |
| disruptive | 4 | 8 | 22 | 36 |
| missense | 4 | 7 | 17 | 30 |
PlasmoDB Total SNPs: 39
Non-coding: 29 | Synonymous: 7 | Nonsynonymous: 3 | Stop Codon: 0
Protein Length: 217 | Molecular Weight (kDa): 24.143
UniProt ID(s): A0A0A0RBM1, A0A0A0RBM2, A0A0A0RCU8, A0A0A0RD04, A0A0A0RD05, A0A0A0RD07, A0A0A0REH4, A0A0A0REH6, A0A0G3FDM9, A0A0N9HJ83, A0A223LV96, A0A223LV98, A0A223LVB0, A0A223LVT0, A0A223LVT2, A0A223LVT8, Q7KQL2, Q9TVS3
PDB ID(s): 7TXW
Isoelectric Point: 6.66
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000742 | EGF-like domain |
| InterPro | IPR010423 | Ookinete surface antigen, EGF domain |
| PFam | PF06247 | Ookinete surface antigen, EGF domain |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16439031 | 3' gene regulatory elements required for expression of the Plasmodiumfalciparumdevelopmental protein, Pfs25. | Oguariri RM, Dunn JM, Golightly LM | 10.1016/j.molbiopara.2005.12.004 |
| 16603573 | Identification and stoichiometry of glycosylphosphatidylinositol-anchoredmembrane proteins of the human malaria parasite Plasmodium falciparum. | Gilson PR, Nebl T, ..., Crabb BS | 10.1074/mcp.M600035-MCP200 |
| 17714601 | Proteins of the malaria parasite sexual stages: expression, function andpotential for transmission blocking strategies. | Pradel G | 10.1017/S0031182007003381 |
| 19032156 | Structure and mechanism of a transmission blocking vaccine candidate proteinPfs25 from P. falciparum: a molecular modeling and docking study. | Sharma B | https://pubmed.ncbi.nlm.nih.gov/19032156/ |
| 23603678 | Alga-produced cholera toxin-Pfs25 fusion proteins as oral vaccines. | Gregory JA, Topol AB, Doerner DZ, Mayfield S | 10.1128/AEM.00714-13 |
| 29029367 | Genetic diversity of Plasmodium falciparum merozoite surface protein-1 (block 2),glutamate-rich protein and sexual stage antigen Pfs25 from Chandigarh, NorthIndia. | Kaur H, Sehgal R, ..., Kanwar JR | 10.1111/tmi.12990 |
| 29146922 | Molecular definition of multiple sites of antibody inhibition of malariatransmission-blocking vaccine antigen Pfs25. | Scally SW, McLeod B, ..., Julien JP | 10.1038/s41467-017-01924-3 |
| 35390042 | Signatures of selection and drivers for novel mutation on transmission-blockingvaccine candidate Pfs25 gene in western Kenya. | Ochwedo KO, Onyango SA, ..., Kazura JW | 10.1371/journal.pone.0266394 |