Genome location: Pf3D7_10_v3:1,256,560..1,258,903(+)
Genome classification: Core
Product Description: MORN repeat-containing protein 1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.1.68 (1-phosphatidylinositol-4-phosphate 5-kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0045178 | basal part of cell |
| Component | GO:0070258 | inner membrane pellicle complex |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.03 | 0.99 |
| Trophozoite | expressed | 0.03 | 0.99 |
| Schizont | expressed | 0.95 | 0.57 |
| Gametocyte | expressed | 0.19 | 0.89 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1031200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.36 | MFS: -2.529 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0515200)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0515200)
Modification: Disrupted | RMgm-2006
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJ93
"Best" AlphaFill ligand hit: CHT (choline ion, Local RMSD=0.02) with 7PL5 (Global RMSD=12.31)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.1.68 | 1-phosphatidylinositol-4-phosphate 5-kinase | ATPADPNEMEDTAPCMBGSSGZnSO4Cutsumheparincysteinesperminequercetin... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q8WTS6 | Histone-lysine N-methyltransferase SETD7 | OrthoMCL BLAST | CHEMBL3238424CHEMBL3414622CHEMBL3414624CHEMBL3414622... |
Protein Length: 364 | Molecular Weight (kDa): 41.45
UniProt ID(s): Q8IJ93
PDB ID(s): None
Isoelectric Point: 5.94
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003409 | MORN motif |
| PFam | PF02493 | MORN motif |
| Superfamily | SSF82185 | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 16 | 10 | 28 |
| disruptive | 3 | 12 | 4 | 21 |
| missense | 3 | 12 | 4 | 19 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 41 | 17 | 23 |
| disruptive | 4 | 41 | 29 | 101 |
| missense | 4 | 33 | 27 | 59 |
PlasmoDB Total SNPs: 142
Non-coding: 130 | Synonymous: 9 | Nonsynonymous: 3 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 18310354 | MORN1 has a conserved role in asexual and sexual development across theapicomplexa. | Ferguson DJ, Sahoo N, ..., Gubbels MJ | 10.1128/EC.00021-08 |
| 31097714 | An essential contractile ring protein controls cell division in Plasmodiumfalciparum. | Rudlaff RM, Kraemer S, Streva VA, Dvorin JD | 10.1038/s41467-019-10214-z |
| 20808817 | A Toxoplasma MORN1 null mutant undergoes repeated divisions but is defective inbasal assembly, apicoplast division and cytokinesis | Lorestani A, Sheiner L, ..., Gubbels MJ | 10.1371/journal.pone.0012302 |
| 35573773 | Toxoplasma gondii's Basal Complex: The Other Apicomplexan Business End IsMultifunctional | Gubbels MJ, Ferguson DJP, ..., Engelberg K | 10.3389/fcimb.2022.882166 |
| 35941170 | Proteomic characterization of the Toxoplasma gondii cytokinesis machineryportrays an expanded hierarchy of its assembly and function | Engelberg K, Bechtel T, ..., Gubbels MJ | 10.1038/s41467-022-32151-0 |
| 34878291 | The Basal Complex Protein PfMORN1 Is Not Required for Asexual Replication ofPlasmodium falciparum | Moran CJ, Dvorin JD | 10.1128/msphere.00895-21 |
| 26763910 | Pellicle formation in the malaria parasite | Kono M, Heincke D, ..., Gilberger TW | 10.1242/jcs.181230 |
| 26318396 | A MORN Repeat Protein Facilitates Protein Entry into the Flagellar Pocket ofTrypanosoma brucei | Morriswood B, Schmidt K | 10.1128/EC.00094-15 |
| 23027733 | Targeted proteomic dissection of Toxoplasma cytoskeleton sub-compartments usingMORN1 | Lorestani A, Ivey FD, ..., Gubbels MJ | 10.1002/cm.21077 |
| 25950173 | Ciprofloxacin Derivatives Affect Parasite Cell Division and Increase the Survivalof Mice Infected with Toxoplasma gondii | Martins-Duarte ES, Dubar F, ..., Vommaro RC | 10.1371/journal.pone.0125705 |
| 37737012 | Characterisation of TbSmee1 suggests endocytosis allows surface-bound cargo toenter the trypanosome flagellar pocket | Schichler D, Konle A, ..., Morriswood B | 10.1242/jcs.261548 |
| 16684814 | A MORN-repeat protein is a dynamic component of the Toxoplasma gondii celldivision apparatus | Gubbels MJ, Vaishnava S, ..., Striepen B | 10.1242/jcs.02949 |
| 26840427 | A MORN1-associated HAD phosphatase in the basal complex is essential forToxoplasma gondii daughter budding | Engelberg K, Ivey FD, ..., Gubbels MJ | 10.1111/cmi.12574 |